Product Detail
Product NameMRCKalpha antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationAffinity purified by Protein A.
ApplicationsWB IHC IF
Species ReactivityHu
Immunogen TypeRecombinant protein
Immunogen DescRecombinant protein fragment contain a sequence corresponding to a region within amino acids 373 and 574 of Human CDC42BPA
Target NameMRCKalpha
ConjugateUnconjugated
Accession NoSwiss-Prot:Q5VT25
Gene ID:8476
Uniprot
Q5VT25
Gene ID
8476;
Concentration2mg/ml
FormulationSupplied in 0.1M Tris-buffered saline with 10% Glycerol (pH7.0). 0.01% Thimerosal was added as a preservative.
StorageStore at -20˚C for long term preservation (recommended). Store at 4˚C for short term use.
Application Details
Predicted MW: 186kd
Western blotting: 1:500-1:3000
Immunohistochemistry: 1:100-1:250
Immunofluorescence: 1:100-1:200
Sample(30 ug of whole cell lysate)
A: H1299
7.5% SDS PAGE
Primary antibody diluted at 1: 1500
Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using MRCK alpha antibody at 1: 100 dilution.
Immunofluorescence analysis of methanol-fixed HCT116, using MRCK alpha antibody at 1: 500 dilution.
The protein encoded by this gene is a member of the Serine/Threonine protein kinase family. This kinase contains multiple functional domains. Its kinase domain is highly similar to that of the myotonic dystrophy protein kinase (DMPK). This kinase also contains a Rac interactive binding (CRIB) domain, and has been shown to bind CDC42. It may function as a CDC42 downstream effector mediating CDC42 induced peripheral actin formation, and promoting cytoskeletal reorganization. Multiple alternatively spliced transcript variants have been described, and the full-length nature of two of them has been reported. [provided by RefSeq]
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Yes
