Product Detail
Product NamePCAF antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationPurified by antigen-affinity chromatography.
ApplicationsWB
Species ReactivityHu
Immunogen TypePeptide
Immunogen DescSynthetic peptide contain a sequence corresponding to a region within amino acids 767 and 832 of PCAF
Target NamePCAF
ConjugateUnconjugated
Accession NoSwiss-Prot:Q92831
Gene ID:8850
Uniprot
Q92831
Gene ID
8850;
Concentration1mg/ml
FormulationSupplied in 0.1M Tris-buffered saline with 10% Glycerol (pH7.0). 0.01% Thimerosal was added as a preservative.
StorageStore at -20˚C for long term preservation (recommended). Store at 4˚C for short term use.
Application Details
Predicted MW: 93kd
Western blotting: 1:500-1:3000
Immunofluorescence: 1:100-1:200
Sample(30 ug whole cell lysate)
A: Hep G2
7.5% SDS PAGE
Primary antibody diluted at 1: 1000
CBP and p300 are large nuclear proteins that bind to many sequence-specific factors involved in cell growth and/or differentiation, including c-jun and the adenoviral oncoprotein E1A. The protein encoded by this gene associates with p300/CBP. It has in vitro and in vivo binding activity with CBP and p300, and competes with E1A for binding sites in p300/CBP. It has histone acetyl transferase activity with core histones and nucleosome core particles, indicating that this protein plays a direct role in transcriptional regulation. [provided by RefSeq]
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Yes
