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JAM-B antibody#22452

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Product Detail

Product NameJAM-B antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationPurified by antigen-affinity chromatography.

ApplicationsWB IHC

Species ReactivityHu

Immunogen TypeRecombinant protein

Immunogen DescRecombinant protein fragment contain a sequence corresponding to a region within amino acids 1 and 264 of Human JAM2

Target NameJAM-B

ConjugateUnconjugated

Accession NoSwiss-Prot:P57087
Gene ID:58494

Uniprot P57087

Gene ID 58494;

Concentration1mg/ml

FormulationSupplied in 0.1M Tris-buffered saline with 10% Glycerol (pH7.0). 0.01% Thimerosal was added as a preservative.

StorageStore at -20˚C for long term preservation (recommended). Store at 4˚C for short term use.

Application Details
Predicted MW: 33kd
Western blotting: 1:500-1:3000
Immunohistochemistry: 1:100-1:250
Sample (30 ug of whole cell lysate)
A: A431
12 % SDS PAGE
Primary antibody diluted at 1: 1000
A:Immunohistochemical analysis of paraffin-embedded TOV-112D xenograft, using JAM-B antibody at 1: 100 dilution. B:Immunohistochemical analysis of paraffin-embedded human colon, using JAM-B antibody at 1: 200 dilution. C:Immunohistochemical analysis of paraffin-embedded mouse small intestine, using JAM-B antibody at 1: 200 dilution.
Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. The protein encoded by this immunoglobulin superfamily gene member is localized in the tight junctions between high endothelial cells. It acts as an adhesive ligand for interacting with a variety of immune cell types and may play a role in lymphocyte homing to secondary lymphoid organs. [provided by RefSeq]

If you have published an article using product 22452, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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