Product Detail
Product NameAP-2M1 antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationPurified by antigen-affinity chromatography.
ApplicationsWB IHC
Species ReactivityHu
Immunogen TypeRecombinant protein
Immunogen DescRecombinant protein fragment contain a sequence corresponding to a region within amino acids 1 and 160 of Human AP2M1
Target NameAP-2M1
ConjugateUnconjugated
Accession NoSwiss-Prot:Q96CW1
Gene ID:1173
Uniprot
Q96CW1
Gene ID
1173;
Concentration0.6mg/ml
FormulationSupplied in 0.1M Tris-buffered saline with 10% Glycerol (pH7.0). 0.01% Thimerosal was added as a preservative.
StorageStore at -20˚C for long term preservation (recommended). Store at 4˚C for short term use.
Application Details
Predicted MW: 50kd
Western blotting: 1:500-1:3000
Immunohistochemistry: 1:100-1:250
Sample(30 ug of whole cell lysate)
A: 293T
B: HeLa S3
10% SDS PAGE
Primary antibody diluted at 1: 500
Immunohistochemical analysis of paraffin-embedded N87 xenograft, using AP2M1 antibody at 1: 100 dilution.
This gene encodes a subunit of the heterotetrameric coat assembly protein complex 2 (AP2), which belongs to the adaptor complexes medium subunits family. The encoded protein is required for the activity of a vacuolar ATPase, which is responsible for proton pumping occurring in the acidification of endosomes and lysosomes. The encoded protein may also play an important role in regulating the intracellular trafficking and function of CTLA-4 protein. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
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Yes
