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Dopamine beta-Hydroxylase antibody#22483

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Product Detail

Product NameDopamine beta-Hydroxylase antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationPurified by antigen-affinity chromatography.

ApplicationsWB IHC IF

Species ReactivityHu

Immunogen TypeRecombinant Protein

Immunogen DescRecombinant protein fragment contain a sequence corresponding to a region within amino acids 8 and 280 (P09172) of Dopamine beta hydroxylase

Target NameDopamine beta-Hydroxylase

ConjugateUnconjugated

Accession NoSwiss-Prot:P09172
Gene ID:1621

Uniprot P09172

Gene ID 1621;

Concentration0.22 mg/ml

FormulationSupplied in 0.1M Tris-buffered saline with 10% Glycerol (pH7.0). 0.01% Thimerosal was added as a preservative.

StorageStore at -20˚C for long term preservation (recommended). Store at 4˚C for short term use.

Application Details

Predicted MW: 69kd
Western blotting: 1:500-1:3000
Immunohistochemistry: 1:100-1:250
Immunofluorescence: 1:100-1:200

Sample (30 ug of whole cell lysate)
A: Hep G2
7.5% SDS PAGE
Primary antibody diluted at 1: 5000
Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using Dopamine beta-Hydroxylase antibody at 1: 500 dilution.
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Dopamine beta-Hydroxylase antibody at 1: 200 dilution.

The protein encoded by this gene is an oxidoreductase belonging to the copper type II, ascorbate-dependent monooxygenase family. It is present in the synaptic vesicles of postganglionic sympathetic neurons and converts dopamine to norepinephrine. It exists in both soluble and membrane-bound forms, depending on the absence or presence, respectively, of a signal peptide. [provided by RefSeq]

If you have published an article using product 22483, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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