Product Detail
Product NameSlap antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationPurified by antigen-affinity chromatography.
ApplicationsWB IF
Species ReactivityHu
Immunogen TypeRecombinant protein
Immunogen DescRecombinant protein fragment contain a sequence corresponding to a region within amino acids 1 and 248 of Slap
Target NameSlap
ConjugateUnconjugated
Accession NoSwiss-Prot:Q13239
Gene ID:6503
Uniprot
Q13239
Gene ID
6503;
Concentration0.9mg/ml
FormulationSupplied in 0.1M Tris-buffered saline with 20% Glycerol (pH7.0). 0.01% Thimerosal was added as a preservative.
StorageStore at -20˚C for long term preservation (recommended). Store at 4˚C for short term use.
Application Details
Predicted MW: 31kd
Western blotting: 1:500-1:3000
Immunofluorescence: 1:100-1:200
Sample (30 ug of whole cell lysate)
A: 293T
B: A431
12% SDS PAGE
Primary antibody diluted at 1: 2000
Immunofluorescence analysis of methanol-fixed A431, using Slap antibody at 1: 200 dilution.
Adapter protein, which negatively regulates T-cell receptor (TCR) signaling. Inhibits T-cell antigen-receptor induced activation of nuclear factor of activated T-cells. Involved in the negative regulation of positive selection and mitosis of T-cells. May act by linking signaling proteins such as ZAP70 with CBL, leading to a CBL dependent degradation of signaling proteins.
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