Product Detail
Product NameDNA ligase 3 antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationPurified by antigen-affinity chromatography.
ApplicationsWB IHC IF
Species ReactivityHu
Immunogen TypeRecombinant protein
Immunogen DescRecombinant protein fragment contain a sequence corresponding to a region within amino acids 765 and 999 of DNA ligase 3
Target NameDNA ligase 3
ConjugateUnconjugated
Accession NoSwiss-Prot:P49916
Gene ID:3980
Uniprot
P49916
Gene ID
3980;
Concentration0.3mg/ml
FormulationSupplied in 1XPBS, 1%BSA, 20% Glycerol (pH7.0). 0.01% Thimerosal was added as a preservative.
StorageStore at -20˚C for long term preservation (recommended). Store at 4˚C for short term use.
Application Details
Predicted MW: 113kd
Western blotting: 1:500-1:3000
Immunohistochemistry: 1:100-1:500
Immunofluorescence: 1:100-1:200
Sample (30 ug of whole cell lysate)
A: Molt-4
7.5% SDS PAGE
Primary antibody diluted at 1: 1000
Immunohistochemical analysis of paraffin-embedded H441 Xenograft, using DNA ligase 3 antibody at 1: 100 dilution.
Immunofluorescence analysis of paraformaldehyde-fixed A431, using DNA ligase 3 antibody at 1: 200 dilution.
This gene is a member of the DNA ligase family. Each member of this family encodes a protein that catalyzes the joining of DNA ends but they each have a distinct role in DNA metabolism. The protein encoded by this gene is involved in excision repair and is located in both the mitochondria and nucleus, with translation initiation from the upstream start codon allowing for transport to the mitochondria and translation initiation from a downstream start codon allowing for transport to the nucleus. Additionally, alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq]
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