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CD55 antibody#22513

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Product Detail

Product NameCD55 antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationPurified by antigen-affinity chromatography.

ApplicationsWB IF

Species ReactivityHu

Immunogen TypeRecombinant protein

Immunogen DescRecombinant protein fragment contain a sequence corresponding to a region within amino acids 71 and 300 of human CD55

Target NameCD55

ConjugateUnconjugated

Accession NoSwiss-Prot:P08174
Gene ID:1604

Uniprot P08174

Gene ID 1604;

Concentration1mg/ml

FormulationSupplied in 0.1M Tris-buffered saline with 10% Glycerol (pH7.0). 0.01% Thimerosal was added as a preservative.

StorageStore at -20˚C for long term preservation (recommended). Store at 4˚C for short term use.

Application Details
Predicted MW: 41kd
Western blotting: 1:500-1:3000
Immunofluorescence: 1:100-1:200
Sample (30 ug of whole cell lysate)
A: THP-1
10% SDS PAGE
Primary antibody diluted at 1: 1000
Immunofluorescence analysis of methanol-fixed HeLa, using CD55 antibody at 1: 200 dilution.
This gene encodes a protein involved in the regulation of the complement cascade. The encoded glycoprotein is also known as the decay-accelerating factor (DAF); binding of DAF to complement proteins accelerates their decay, disrupting the cascade and preventing damage to host cells. Antigens present on the DAF glycoprotein constitute the Cromer blood group system (CROM). Two alternatively spliced transcripts encoding different proteins have been identified. The predominant transcript encodes a membrane-bound protein expressed on cells exposed to plasma component proteins but an alternatively spliced transcript produces a soluble protein present at much lower levels. Additional, alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq]

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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