Product Detail
Product NameSAP130 antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationPurified by antigen-affinity chromatography.
ApplicationsWB IHC
Species ReactivityHu
Immunogen TypePeptide
Immunogen DescSynthetic peptide contain a sequence corresponding to a region within amino acids 1154 and 1217 of SAP130
Target NameSAP130
ConjugateUnconjugated
Accession NoSwiss-Prot:Q15393
Gene ID:23450
Uniprot
Q15393
Gene ID
23450;
Concentration1mg/ml
FormulationSupplied in 1XPBS, 10% Glycerol (pH7.0). 0.01% Thimerosal was added as a preservative.
StorageStore at -20˚C for long term preservation (recommended). Store at 4˚C for short term use.
Application Details
Predicted MW: 136kd
Western blotting: 1:500-1:3000
Immunohistochemistry: 1:100-1:250
Sample (30 ug of whole cell lysate)
A: Hela
5% SDS PAGE
Primary antibody diluted at 1: 3000
Immunohistochemical analysis of paraffin-embedded Saos-2 xenograft, using SF3B3 antibody at 1: 100 dilution.
This gene encodes subunit 3 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. Subunit 3 has also been identified as a component of the STAGA (SPT3-TAF(II)31-GCN5L acetylase) transcription coactivator-HAT (histone acetyltransferase) complex, and the TFTC (TATA-binding-protein-free TAF(II)-containing complex). These complexes may function in chromatin modification, transcription, splicing, and DNA repair. [provided by RefSeq]
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Yes
