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Histamine H2 Receptor antibody#22532

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Product Detail

Product NameHistamine H2 Receptor antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationPurified by antigen-affinity chromatography.

ApplicationsWB IHC IF

Species ReactivityHu

Immunogen TypePeptide

Immunogen DescSynthetic peptide contain a sequence corresponding to a region within amino acids 1 and 11 (P25021) of Histamine H2 Receptor

Target NameHistamine H2 Receptor

ConjugateUnconjugated

Other NamesH2R

Accession NoSwiss-Prot:P25021
Gene ID:3274

Uniprot P25021

Gene ID 3274;

Concentration0.75mg/ml

FormulationSupplied in 0.1M Tris-buffered saline with 10% Glycerol (pH7.0). 0.01% Thimerosal was added as a preservative.

StorageStore at -20˚C for long term preservation (recommended). Store at 4˚C for short term use.

Application Details

Predicted MW: 40kd
Western blotting: 1:500-1:3000
Immunohistochemistry: 1:50-1:500
Immunofluorescence: 1:100-1:200

Sample (30 ug of whole cell lysate)
A: Hela
10% SDS PAGE
Primary antibody diluted at 1: 1000
Immunohistochemical analysis of paraffin-embedded H520 xenograft, using Histamine H2 Receptor antibody at 1: 100 dilution.
Immunofluorescence analysis of methanol-fixed HeLa, using Histamine H2 Receptor antibody at 1: 200 dilution.

Histamine is a ubiquitous messenger molecule released from mast cells, enterochromaffin-like cells, and neurons. Its various actions are mediated by histamine receptors H1, H2, H3 and H4. Histamine receptor H2 belongs to the family 1 of G protein-coupled receptors. It is an integral membrane protein and stimulates gastric acid secretion. It also regulates gastrointestinal motility and intestinal secretion and is thought to be involved in regulating cell growth and differentiation. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

If you have published an article using product 22532, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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