Product Detail
Product NameCollagen I alpha2 antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationPurified by antigen-affinity chromatography.
ApplicationsWB IF
Species ReactivityHu
Immunogen TypeRecombinant protein
Immunogen DescRecombinant protein fragment contain a sequence corresponding to a region within amino acids 1057 and 1339 of Collagen I alpha2
Target NameCollagen I alpha2
ConjugateUnconjugated
Accession NoSwiss-Prot:P08123
Gene ID:1278
Uniprot
P08123
Gene ID
1278;
Concentration1mg/ml
FormulationSupplied in 0.1M Tris-buffered saline with 10% Glycerol (pH7.0). 0.01% Thimerosal was added as a preservative.
StorageStore at -20˚C for long term preservation (recommended). Store at 4˚C for short term use.
Application Details
Predicted MW: 129kd
Western blotting: 1:500-1:3000
Immunofluorescence: 1:100-1:200
Sample (30 ug of whole cell lysate)
A: 293T
B: A431
7.5% SDS PAGE
Primary antibody diluted at 1: 1000
Immunofluorescence analysis of paraformaldehyde-fixed A431, using Collagen I alpha2 antibody at 1: 200 dilution.
This gene encodes the pro-alpha2 chain of type I collagen whose triple helix comprises two alpha1 chains and one alpha2 chain. Type I is a fibril-forming collagen found in most connective tissues and is abundant in bone, cornea, dermis and tendon. Mutations in this gene are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIB, recessive Ehlers-Danlos syndrome Classical type, idiopathic osteoporosis, and atypical Marfan syndrome. Symptoms associated with mutations in this gene, however, tend to be less severe than mutations in the gene for the alpha1 chain of type I collagen (COL1A1) reflecting the different role of alpha2 chains in matrix integrity. Three transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene. [provided by R. Dalgleish]
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