Product Detail
Product NameSEPHS2 antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationPurified by antigen-affinity chromatography.
ApplicationsWB IF
Species ReactivityHu
Immunogen TypeRecombinant protein
Immunogen DescRecombinant protein fragment contain a sequence corresponding to a region within amino acids 186 and 422 of SEPHS2
Target NameSEPHS2
ConjugateUnconjugated
Accession NoSwiss-Prot:Q99611
Gene ID:22928
Uniprot
Q99611
Gene ID
22928;
Concentration1mg/ml
FormulationSupplied in 0.1M Tris-buffered saline with 10% Glycerol (pH7.0). 0.01% Thimerosal was added as a preservative.
StorageStore at -20˚C for long term preservation (recommended). Store at 4˚C for short term use.
Application Details
Predicted MW: 47kd
Western blotting: 1:500-1:3000
Immunofluorescence: 1:100-1:200
Sample (30ug whole cell lysate)
A: HeLa S3
7.5% SDS PAGE
Primary antibody diluted at 1: 1000
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using SEPHS2 antibody at 1: 200 dilution.
This gene encodes an enzyme that synthesizes selenophosphate from selenide and ATP. Selenophosphate is the selenium donor used to synthesize selenocysteine, which is co-translationally incorporated into selenoproteins at in-frame UGA codons. This protein itself contains a selenocysteine residue in its predicted active site. The 3' UTR of the gene has a stem-loop secondary structure called a selenocysteine insertion sequence (SECIS) element, which allows UGA to direct the incorporation of selenocysteine rather than signal a translational stop. Alternatively spliced transcripts have been identified, but their biological validity has not been determined. [provided by RefSeq]
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