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Beta Actin antibody#22957

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Product Detail

Product NameBeta Actin antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationPurified by antigen-affinity chromatography.

ApplicationsWB IHC IF

Species ReactivityHu

Immunogen TypePeptide

Immunogen DescA synthesized peptide derived from human beta Actin

Target NameBeta Actin

ConjugateUnconjugated

Accession NoSwiss-Prot:P60709
Gene ID:60

Uniprot P60709

Gene ID 60;

Concentration1mg/ml

FormulationRabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

StorageStore at -20˚C for long term preservation (recommended). Store at 4˚C for short term use.

Application Details

Predicted MW: 42kd
Western blotting: 1:500-1:3000
Immunohistochemistry: 1:100-1:250
Immunofluorescence: 1:100-1:200
 

Sample (30 ug of whole cell lysate)
A: A431
B: H1299
10% SDS PAGE
Primary antibody diluted at 1: 1000
Immunohistochemical analysis of paraffin-embedded Prostate, using beta Actin antibody(10 ug/ml).
Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using beta Actin antibody (Green) at 1: 500 dilution and alpha-tubulin antibody (Red) at 1: 2000.

This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. [provided by RefSeq]

If you have published an article using product 22957, please notify us so that we can cite your literature.

et al,Tumor cell membrane enveloped aluminum phosphate nanoparticles for enhanced cancer vaccination , (2020),
PMID: 32659330
et al,Effect of silencing CD147 on glycolysis in prostate cancer LNCaP cells , (2023),
PMID:
et al,Bioinformatics and validation reveal the potential target of curcumin in the treatment of diabetic peripheral neuropathy , (2024),
PMID:

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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