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Alpha-tubulin Antibody#24864

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Product Detail

Product NameAlpha-tubulin Antibody

ClonalityPolyclonal

PurificationAffinity chromatography purified via peptide column

ApplicationsELISA WB

Species ReactivityHu Ms Rt

Immunogen TypePeptide

Immunogen DescRaised against a 16 amino acid peptide near the amino terminus of human Tubulin.

Target NameAlpha-tubulin

ConjugateUnconjugated

Other NamesAlpha-tubulin, Tubulin alpha-1A, TUBA1A, TUBA3, LIS3

Accession NoSwiss-Prot:Q13748
Gene ID:113457

Uniprot Q13748

Gene ID 113457;

Concentration1mg/ml

FormulationSupplied in PBS containing 0.02% sodium azide.

StorageAntibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.

Application Details
Western blot analysis of Tubulin in rat brain tissue lysate with Tubulin antibody at (A) 0.5 and (B) 1 ug/mL.

Alpha-tubulin belongs to the tubulin superfamily, which is composed of six distinct families. Along with beta-tubulins, alpha-tubulins are the major components of microtubules. These microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Alpha- and beta-tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22-nm diameter. Tyrosine ligase adds a C-terminal tyrosine to monomeric alpha-tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton-associated carboxypeptidase. Another post-translational modification of detyrosinated alpha-tubulin is C-terminal polyglutamylation, which is characteristic of microtubules in neuronal cells and the mitotic spindle. Like GAPDH and beta-Actin, this antibody makes an excellent loading control in immunoblots.

If you have published an article using product 24864, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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