Product Detail
Product NamePUMA Monoclonal Antibody
Clone No.mAb (Clone 10D4G7)
Host SpeciesMouse
ClonalityMonoclonal
PurificationImmunoaffinity chromotography purified IgG
ApplicationsELISA WB
Species ReactivityHu Rt
Immunogen TypeRecombinant protein
Immunogen DescRecombinant protein corresponding to amino acids 76 - 170 of human PUMA-alpha.
Target NamePUMA
ConjugateUnconjugated
Other NamesPUMA (10D4G7), p53 upregulated modulator of apoptosis, bbc3, Bcl-2 binding component 3
Accession NoQ9BXH1
Uniprot
Q96PG8
Gene ID
27113;
Concentration1mg/ml
FormulationSupplied in PBS containing 0.02% sodium azide.
StorageCan be stored at -20˚C, stable for one year.
Application Details
Western blot analysis of PUMA expression in K562 cell lysate with PUMA antibody at (A) 2.5 and (B) 5 ug/mL.
Apoptosis is related to many diseases and development. The p53 tumor-suppressor protein induces apoptosis through transcriptional activation of several genes. A novel p53 inducible pro-apoptotic gene was identified recently and designated PUMA (for p53 upregulated modulator of apoptosis) and bbc3 (for Bcl-2 binding component 3) in human and mouse. PUMA/bbc3 is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53. The PUMA gene encodes two BH3 domain-containing proteins termed PUMAα and PUMAβ. PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis.
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