Product Detail
Product NameEndoG Monoclonal Antibody
Clone No.mAb (Clone 7G1G10)
Host SpeciesMouse
ClonalityMonoclonal
PurificationImmunoaffinity chromotography purified IgG
ApplicationsELISA WB
Species ReactivityHu Rt
Immunogen TypeRecombinant protein
Immunogen DescRecombinant protein corresponding to amino acids 51 - 140 of human EndoG.
Target NameEndoG
ConjugateUnconjugated
Other NamesEndoG (7G1G10): Endonuclease G
Accession NoQ14249
Uniprot
Q14249
Gene ID
2021;
Concentration1mg/ml
FormulationSupplied in PBS containing 0.02% sodium azide.
StorageCan be stored at -20˚C, stable for one year.
Application Details
Western blot analysis of EndoG expression in HepG2 cell lysate with EndoG antibody at (A) 5 and (B) 10 ug/mL.
The fragmentation of nuclear DNA is a hallmark of apoptotic cell death. The activities of caspase and nuclease are involved in the DNA fragmentation. Caspase-activated deoxyribonuclease (CAD), also termed DNA fragmentation factor (DFF40), is one such nuclease, and is capable of inducing DNA fragmentation and chromatin condensation after cleavage by caspase-3 of its inhibitor ICAD/DFF45. Caspase and CAD independent DNA fragmentation also exists. Recent studies demonstrated that another nuclease, endonuclease G (EndoG), is specifically activated by apoptotic stimuli and is able to induce nucleosomal fragmentation of DNA independently of caspase and DFF/CAD. EndoG is a mitochondrion-specific nuclease that translocates to the nucleus and cleaves chromatin DNA during apoptosis. The homologue of mammalian EndoG is the first mitochondrial protein identified to be involved in apoptosis in C. elegans. EndoG also cleaves DNA in vitro.
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