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CD4 Monoclonal Antibody#26037

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Product Detail

Product NameCD4 Monoclonal Antibody

Clone No.mAb (Clone 8G1B12)

Host SpeciesMouse

ClonalityMonoclonal

PurificationImmunoaffinity chromotography purified IgG

ApplicationsELISA WB

Species ReactivityHu Ms Rt

Immunogen TypeRecombinant protein

Immunogen DescRaised against a 193 amino acid recombinant protein from near the amino terminus of human CD4.

Target NameCD4

ConjugateUnconjugated

Other NamesCD4 (8G1B12), CD4 antigen, CD4 receptor

Accession NoSwiss-Prot:P01730
Gene ID:920

Uniprot P01730

Gene ID 920;

Concentration1mg/ml

FormulationSupplied in PBS containing 0.02% sodium azide.

StorageCan be stored at -20˚C, stable for one year.

Application Details
Western blot analysis of CD4 in rat lung tissue lysate with CD4 antibody at (A) 0.5 and (B) 1 ug/mL.
Immunofluorescence of CD4 in human thymus tissue with CD4 antibody at 5 μg/ml.
Immunohistochemistry of CD4 in human thymus tissue with CD4 antibody at 5 μg/ml.
CD4 is a member of the immunoglobulin superfamily and is implicated as associative recognition element in MHC (Major Histocompatibility Complex) class II-restricted immune response. On T-lymphocytes, it defines the helper/inducer subset. The mature 55 kd CD4 protein consists of a 372 amino acid extracellular segment composed of four tandem immunoglobulin-like VJ regions. The CD4 molecule is a major receptor for human immunodeficiency virus (HIV), binding directly to the envelope glycoprotein gp120 on HIV, with the co-receptors being CCR5 or CXCR4. It has been shown that the V-like domains are critical for binding with HIV envelope gp120.

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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