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SMAD5(C-term) Monoclonal Antibody#27047

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Product Detail

Product NameSMAD5(C-term) Monoclonal Antibody

Host SpeciesMouse

ClonalityMonoclonal

IsotypeIgG1

PurificationPurified by antigen-affinity chromatography.

ApplicationsWB ICC FC

Species ReactivityHu P Rt Ms

Immunogen TypeRecombinant protein

Immunogen DescRecombinant fragment, corresponding to amino acids 239aa-372aa of human SMAD5 (C-terminus).

Target NameSMAD5

ConjugateUnconjugated

Other Namesmothers against decapentaplegic homolog 5 SMA- and MAD-related protein 5 SMAD, mothers against DPP homolog 5 MAD, mothers against decapentaplegic homolog 5 mothers against decapentaplegic, drosophila, homolog of, 5

Accession NoUniProt:Q99717
NCBI Gene ID:4090

Uniprot Q99717

Gene ID 4090;

FormulationPurified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50%,glycerol

Storagestore at -20˚C

Application Details

Predicted MW: 52kd
Western blotting: 1:1000

1:1000 dilution of this antibody detected SMAD5 on 40ug of cell lysates
HeLa cells using anti- SMAD5 (C-terminus) antibody diluted 1:150; Flow Cytometry analysis Jurkat cells stained with SMAD5 (red, 1:100 dilution), followed by FITC-conjugated goat anti-mouse IgG. Black line histogram represents the isotype control, normal m
Jurkat cells stained with SMAD5 (red, 1:100 dilution), followed by FITC-conjugated goat anti-mouse IgG. Black line histogram represents the isotype control, normal mouse IgG

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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