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Dynactin 1(N-terminus) Monoclonal Antibody#27082

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Product Detail

Product NameDynactin 1(N-terminus) Monoclonal Antibody

Clone No.3D5-C6-D5

Host SpeciesMouse

ClonalityMonoclonal

IsotypeIgG2b

PurificationAffinity purified

ApplicationsELISA WB ICC

Species ReactivityHu P Ms B

Immunogen TypeRecombinant protein

Immunogen DescPurified recombinant human DCTN1 protein fragments expressed in E.coli.

Target NameDynactin 1

ConjugateUnconjugated

Other NamesP135, DP-150, DAP-150

Accession NoSwiss-Prot:Q14203
Gene ID:1639

Uniprot Q14203

Gene ID 1639;

FormulationPurified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50%,glycerol

Application Details
Predicted MW: 150kd
Western blotting: 1:1000
Immunocytochemistry:1:300
Western blot detection of DCTN1 in A2780,TF-1,HepG2,MCF7,A431&K562 cell lysate(1:1000 diluted).
Predicted band size:150KDa
Observed band size: 150KDa
Required for the cytoplasmic dynein-driven retrograde movement of vesicles and organelles along microtubules. Dynein-dynactin interaction is a key component of the mechanism of axonal transport of vesicles and organelles.

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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