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DLAT Monoclonal Antibody#27119

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Product Detail

Product NameDLAT Monoclonal Antibody

Clone No.4A4-B6-C10

Host SpeciesMouse

ClonalityMonoclonal

IsotypeIgG1

PurificationAffinity purified

ApplicationsELISA WB ICC IP

Species ReactivityHu Ms

Immunogen TypeRecombinant Protein

Immunogen DescPurified recombinant human DLAT protein fragments expressed in E.coli.

Target NameDLAT

ConjugateUnconjugated

Other NamesDLTA, PDCE2, PDC-E2

Accession NoSwiss-Prot:O15379
Gene ID:8841

Uniprot O15379

Gene ID 8841;

FormulationPurified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50%,glycerol

Application Details

Predicted MW: 69kd
Western blotting: 1:1000
Immunocytochemistry:1:300

Western blot detection of DLAT in Jurkat,A549,U251,F9,LNCAP&Hela cell lysates using DLAT antibody (1:1000 diluted).
Predicted band size:69KDa.
Observed band size:69KDa
Immunocytochemistry stain of Hela using DLAT antibody (1:300).
Immunoprecipitation analysis of Hela cell lysates using DLAT antibody

This gene encodes component E2 of the multi-enzyme pyruvate dehydrogenase complex (PDC). PDC resides in the inner mitochondrial membrane and catalyzes the conversion of pyruvate to acetyl coenzyme A. The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO2. It contains multiple copies of three enzymatic components.

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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