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PGC-1 alpha Monoclonal Antibody #27135

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Product Detail

Product NamePGC-1 alpha Monoclonal Antibody

Host SpeciesMouse

ClonalityMonoclonal

PurificationAffinity-chromatography

ApplicationsELISA WB

Species ReactivityHu

SpecificityPGC-1alpha antibody detects endogenous levels of total PGC-1alpha

Immunogen TypeRecombinant Protein

Immunogen DescPurified recombinant fragment of human PGC-1alpha expressed in E. Coli

Target NamePGC-1 alpha

ConjugateUnconjugated

Other NamesPPARGC1A; LEM6; PGC1; PGC1A; PGC-1v; PPARGC1; PGC-1(alpha)

Accession NoSwiss-Prot#: Q9UBK2

Uniprot Q9UBK2

Gene ID 10891;

Sdspage MW91KD

Concentration1.0mg/ml

FormulationMouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt

StorageStore at -20°C/1 year

Application Details
 ELISA: 1:10000 
 WB: 1:500 - 1:2000
Western blot analysis using PGC-1alpha mAb against human PGC-1alpha (AA: 570-798) recombinant protein. (Expected MW is 70 kDa)

The protein encoded by this gene is a transcriptional coactivator that regulates the genes involved in energy metabolism. This protein interacts with PPAR gamma, which permits the interaction of this protein with multiple transcription factors. This protein can interact with, and regulate the activities of, cAMP response element binding protein (CREB) and nuclear respiratory factors (NRFs). It provides a direct link between external physiological stimuli and the regulation of mitochondrial biogenesis, and is a major factor that regulates muscle fiber type determination. This protein may be also involved in controlling blood pressure, regulating cellular cholesterol homoeostasis, and the development of obesity. 

If you have published an article using product 27135, please notify us so that we can cite your literature.

et al,Helpful to Live Healthier? Intermittent Hypoxic/Ischemic Training Benefits Vascular Homeostasis and Lipid Metabolism with Activating SIRT1 Pathways in Overweight/Obese Individuals , (2024),
PMID:
et al,Classical prescription Daqinjiao decoction inhibit cerebral ischemia/reperfusion induced necroptosis and ferroptosis through multiple mechanisms. , (2025),
PMID: 39736347

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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