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HP1-gamma Monoclonal Antibody#27171

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Product Detail

Product NameHP1-gamma Monoclonal Antibody

Clone No.5G10-F7-A12

Host SpeciesMouse

ClonalityMonoclonal

IsotypeIgG1

PurificationAffinity purified

ApplicationsWB ICC IP IHC

Species ReactivityMs Hu Mk Hm Rt

SpecificityThis antibody detects endogenous levels of HP1-gamma and does not cross-react with related proteins.

Immunogen TypeRecombinant Protein

Immunogen DescPurified recombinant human HP1-gamma protein fragments expressed in E.coli.

Target NameHP1-gamma

ConjugateUnconjugated

Other NamesCBX 3; CBX3; CBX3_HUMAN; Chromobox homolog 3 (HP1 gamma homolog, Drosophila); Chromobox homolog 3; Chromobox protein homolog 3; GAMMA; HECH; Heterochromatin like protein 1; Heterochromatin protein 1 homolog gamma; Heterochromatin protein HP1 gamma

Accession NoUniprot: Q13185

Gene ID: 11335

Uniprot Q13185

Gene ID 11335;

Sdspage MW22kd

FormulationPurified mouse monoclonal antibody in PBS(pH 7.4) containing with 0.02% sodium azide and 50% glycerol.

Storagestore at -20˚C

Application Details

Western blotting: 1:1000

Immunocytochemistry: 1:200
Immunocytochemistry staining of HeLa cells fixed with 4% Paraformaldehyde and using anti-HP1-gamma antibody (dilution 1:200).
Western blot detection of HP1-gamma in Hela,3T3,C6,COS7 and CHO-K1 cell lysates using HP1-gamma antibody (1:1000 diluted).Predicted band size:22KDa.Observed band size:22KDa.
Immunoprecipitation analysis of Hela cell lysates using HP1-gamma antibody.
Immunohistochemical analysis of paraffin-embedded Prostate Cancer using HP1-gamma Mouse mAb (1/200 dilution).Antigen retrieval was performed by pressure cooking in citrate buffer (pH 6.0).

 Seems to be involved in transcriptional silencing in heterochromatin-like complexes. Recognizes and binds histone H3 tails methylated at 'Lys-9', leading to epigenetic repression. May contribute to the association of the heterochromatin with the inner nuclear membrane through its interaction with lamin B receptor (LBR). Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins.

If you have published an article using product 27171, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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