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CD54(ICAM-1) Monoclonal Antibody#27176

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Product NameCD54(ICAM-1) Monoclonal Antibody

Clone No.4F5-F9-F12

Host SpeciesMouse

ClonalityMonoclonal

IsotypeIgG2b

PurificationAffinity purified

ApplicationsWB ICC

Species ReactivityHu

SpecificityThis antibody detects endogenous levels of ICAM1(C-terminus) and does not cross-react with related proteins.

Immunogen TypeRecombinant Protein

Immunogen DescPurified recombinant human ICAM1(C-terminus) protein fragments expressed in E.coli.

Target NameCD54(ICAM-1)

ConjugateUnconjugated

Other NamesBB 2; BB2; CD 54; CD_antigen=CD54; CD54; Cell surface glycoprotein P3.58; Human rhinovirus receptor; ICAM 1; ICAM-1; ICAM1; ICAM1_HUMAN; Intercellular adhesion molecule 1; Major group rhinovirus receptor; MALA 2; MALA2; MyD 10; MyD10; P3.58

Accession NoUniprot: P05362

Gene ID: 3383

Uniprot P05362

Gene ID 3383;

Sdspage MW96kd

FormulationPurified mouse monoclonal in PBS(pH 7.4) containing with 0.02% sodium azide and 50% glycerol.

Storagestore at -20˚C

Application Details

Western blotting: 1:1000

Immunocytochemistry: 1:200
Immunocytochemistry staining of SW480 cells fixed by anhydrous methanol for 2 h at -20℃ and using anti-ICAM1 antibody (dilution 1:200).
Western blot detection of ICAM1 antibody in SW480 and Raji cell lysates using ICAM1 antibody (1:1000 diluted).Predicted band size:96KDa.Observed band size:96KDa.

 ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. In case of rhinovirus infection acts as a cellular receptor for the virus.

If you have published an article using product 27176, please notify us so that we can cite your literature.

et al,Astragalus polysaccharides mediated preventive effects on bronchopulmonary dysplasia in rats.In Pediatr Res on 2014 Oct by Xiao-Hong Wang, Hong-Ling Jia et al..PMID: 25029259 , (2014),
PMID: 25029259

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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