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Keratin 18 Monoclonal Antibody#27178

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Product Detail

Product NameKeratin 18 Monoclonal Antibody

Clone No.2B5-B6-F7

Host SpeciesMouse

ClonalityMonoclonal

IsotypeIgG2a

PurificationAffinity purified

ApplicationsWB ICC

Species ReactivityHu Mk

SpecificityThis antibody detects endogenous levels of krt18 and does not cross-react with related proteins.

Immunogen TypeRecombinant Protein

Immunogen DescPurified recombinant human KRT18 protein fragments expressed in E.coli.

Target NameKeratin 18

ConjugateUnconjugated

Other NamesCell proliferation-inducing gene 46 protein; Cytokeratin-18; Keratin-18; krt18;

Accession NoUniprot: P05783

Gene ID: 3875

Uniprot P05783

Gene ID 3875;

Sdspage MW46kd

FormulationPurified mouse monoclonal in PBS(pH 7.4) containing with 0.02% sodium azide,0.1mg/mlBSA and 50% glycerol.

Storagestore at -20˚C

Application Details

Western blotting: 1:2000

Immunocytochemistry: 1:800
Immunocytochemistry staining of HeLa cells fixed by anhydrous methanol for 2 h at -20℃ and using anti-krt18 antibody (dilution 1:800).
Western blot detection of krt18 antibody in Hela,COS7 and 293T cell lysates using krt18 antibody (1:2000 diluted).Predicted band size:46KDa.Observed band size:46KDa.

 Involved in the uptake of thrombin-antithrombin complexes by hepatic cells By similarity. When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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