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Location： Home > Mouse Monoclonal Antibodies > CSK Monoclonal Antibody

CSK Monoclonal Antibody#27181

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Product Detail

Product NameCSK Monoclonal Antibody

Clone No.2B4-C6-A10

Host SpeciesMouse

ClonalityMonoclonal

IsotypeIgG2b

PurificationAffinity purified

ApplicationsWB ICC

Species ReactivityHu Rt

SpecificityThis antibody detects endogenous levels of CSK and does not cross-react with related proteins.

Immunogen TypeRecombinant Protein

Immunogen DescPurified recombinant human CSK protein fragments expressed in E.coli.

Target NameCSK

ConjugateUnconjugated

Other NamesC SRC; C SRC kinase; C src Tyrosine Kinase; C-SRC kinase; c-src tyrosine kinase; Csk A; CSK; CSK_HUMAN; CYTOPLASMIC TYROSINE KINASE; EC2.7.10.2; MGC112926; MGC117393; MGC154049; P60 Src; Protein tyrosine kinase CYL; Protein-tyrosine kinase CYL;

Accession NoUniprot: P41240

Gene ID: 1445

Uniprot P41240

Gene ID 1445;

Sdspage MW50kd

FormulationPurified mouse monoclonal in buffer containing 0.1M Tris-Glycine(pH 7.4,150 mM NaCl)with 0.2% sodium azide,0.1mg/mlBSA and 50% glycerol.

Storagestore at -20˚C

Application Details

Western blotting: 1:1000

Immunocytochemistry: 1:50
Immunocytochemistry staining of C6 cells fixed by anhydrous methanol for 2 h at -20℃ and using anti-CSK antibody (dilution 1:50).
Western blot detection of CSK antibody in C6 and Jurkat cell lysates using CSK antibody (1:1000 diluted).Predicted band size:50KDa.Observed band size:50KDa.

 Non-receptor tyrosine-protein kinase that plays an important role in the regulation of cell growth, differentiation, migration and immune response. Phosphorylates tyrosine residues located in the C-terminal tails of Src-family kinases (SFKs) including LCK, SRC, HCK, FYN, LYN or YES1. Upon tail phosphorylation, Src-family members engage in intramolecular interactions between the phosphotyrosine tail and the SH2 domain that result in an inactive conformation. To inhibit SFKs, CSK is recruited to the plasma membrane via binding to transmembrane proteins or adapter proteins located near the plasma membrane. Suppresses signaling by various surface receptors, including T-cell receptor (TCR) and B-cell receptor (BCR) by phosphorylating and maintaining inactive several positive effectors such as FYN or LCK.

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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