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DNA-PKcs Monoclonal Antibody#27182

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Product Detail

Product NameDNA-PKcs Monoclonal Antibody

Clone No.8D3-B12-F11

Host SpeciesMouse

ClonalityMonoclonal

IsotypelgG2b

PurificationAffinity purified

ApplicationsWB IP ICC

Species ReactivityHu

SpecificityThis antibody detects endogenous levels of DNA-PKcs,and does not cross-react with related proteins.

Immunogen TypeRecombinant Protein

Immunogen DescPurified recombinant human DNA-PKcs protein fragments expressed in E.coli

Target NameDNA-PKcs

ConjugateUnconjugated

Other NamesDNA dependent protein kinase catalytic subuni; DNA PKcs; DNA-dependent protein kinase catalytic subunit; DNA-PK catalytic subunit; DNA-PKcs; DNAPK; DNPK1; hyper radiosensitivity of murine scid mutation, complementing 1; HYRC; HYRC1; p350; p460; PRKDC;

Accession NoUniprot: P78527

Gene ID: 5591

Uniprot P78527

Gene ID 5591;

Sdspage MW450kd

FormulationPurified mouse monoclonal in PBS(pH 7.4)containing with 0.2% sodium azide,50% glycerol.

Storagestore at -20˚C

Application Details

Western blotting: 1:1000

Immunocytochemistry: 1:200
Immunocytochemistry stain of Hela using PRKDC antibody (1:200).
Immunoprecipitation analysis of Hela cell lysates using PRKDC antibody
Western blot detection of PRKDC in Hela,K562,HL-60&MOLT-4 cell lysates using PRKDC antibody(1:1000 diluted).Predicted band size:450KDa,Observed band size:450KDa
Western blot detection of PRKDC in K562,Molt-4,A549 and HCT116 cell lysates using PRKDC antibody(1:1000 diluted).Predicted band size:450KDa.Observed band size:450KDa.

 properties.Promotes processing of hairpin DNA structures in V(D)J recombination by activation of the hairpin endonuclease artemis(DCLRE1C).The assembly of the DNA-PK complex at DNA ends is also required for the NHEJ ligation step.Required to protect and align broken ends of DNA.May also act as a scaffold protein to aid the localization of DNA repair proteins to the site of damage.Found at the ends of chromosomes,suggesting a further role in the maintenance of telomeric stability and the prevention of chromosomal end fusion.Also involved in modulation of transcription.Recognizes the substrate consensus sequence[ST]-Q.Phosphorylates'Ser-139' of histone variant H2AX/H2AFX,thereby regulating DNA damage response mechanism.Phosphorylates DCLRE1C,c-Abl/ABL1,histone H1,HSPCA,c-jun/JUN,p53/TP53, PARP1, POU2F1, DHX9, SRF, XRCC1, XRCC1, XRCC4, XRCC5, XRCC6, WRN, MYC and RFA2.Can phosphorylate C1D not only in the presence of linear DNA but also in the presence of supercoiled DNA.Ability to phosphorylate p53/TP53 in the presence of supercoiled DNA is dependent on C1D.

If you have published an article using product 27182, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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