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Location： Home > Mouse Monoclonal Antibodies > UCHL1/PGP9.5 Monoclonal Antibody

UCHL1/PGP9.5 Monoclonal Antibody#27188

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Product NameUCHL1/PGP9.5 Monoclonal Antibody

Clone No.7B8-G5-E7

Host SpeciesMouse

ClonalityMonoclonal

IsotypeIgG2b

PurificationAffinity purified

ApplicationsWB ICC

Species ReactivityHu

SpecificityThis antibody detects endogenous levels of UCHL1 and does not cross-react with related proteins.

Immunogen TypeRecombinant Protein

Immunogen DescPurified recombinant human UCHL1. protein fragments expressed in E.coli.

Target NameUCHL1/PGP9.5

ConjugateUnconjugated

Other NamesNeuron cytoplasmic protein 9.5; PGP 9.5; Ubiquitin thioesterase L1

Accession NoUniprot: P09936

Gene ID: 7345

Uniprot P09936

Gene ID 7345;

Sdspage MW25kd

FormulationPurified mouse monoclonal in PBS(pH 7.4) containing with 0.2% sodium azide, 50% glycerol, 0.1%BSA

Storagestore at -20˚C

Application Details

Western blotting: 1:1000

Immunocytochemistry: 1:300
Immunocytochemistry stain of COS7 using UCHL1 antibody (1:300).
Western blot detection of UCHL1 in U251,Raji&IMP32 cell lysates and using UCHL1 antibody (1:1000 diluted). Predicted band size: 25KDa Observed band size:25KDa

 Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity

 

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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