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Placental alkaline phosphatase (PLAP) Monoclonal Antibody#27192

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Product NamePlacental alkaline phosphatase (PLAP) Monoclonal Antibody

Clone No.8F10-2C10-C9

Host SpeciesMouse

ClonalityMonoclonal

IsotypeIgG2b

PurificationAffinity purified

ApplicationsWB ICC

Species ReactivityHu

SpecificityThis antibody detects endogenous levels of ALPP and does not cross-react with related proteins.

Immunogen TypeRecombinant Protein

Immunogen DescPurified recombinant human ALPP protein fragments expressed in E.coli.

Target NamePlacental alkaline phosphatase(PLAP)

ConjugateUnconjugated

Other NamesAlkaline phosphatase Regan isozyme; Placental alkaline phosphatase 1;

Accession NoUniprot: P05187

Gene ID: 250

Uniprot P05187

Gene ID 250;

Sdspage MW76kd

FormulationPurified mouse monoclonal in PBS(pH 7.4) containing with 0.2% sodium azide, 50% glycerol, 0.1%BSA

Storagestore at -20˚C

Application Details
Western blotting: 1:500
Immunocytochemistry: 1:300
Immunocytochemistry stain of Hela using ALPP antibody (1:300).
Western blot detection of ALPP in Hela,LNCap,human Placenta &A549 cell lysates and using ALPP antibody (1:500 diluted). Predicted band size: 76KDa Observed band size:76KDa

 There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2 while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme, also referred to as the heat stable form, that is expressed primarily in the placenta although it is closely related to the intestinal form of the enzyme as well as to the placental-like form. The coding sequence for this form of alkaline phosphatase is unique in that the 3' untranslated region contains multiple copies of an Alu family repeat. In addition, this gene is polymorphic and three common alleles (type 1, type 2 and type 3) for this form of alkaline phosphatase have been well characterized.

If you have published an article using product 27192, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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