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Location： Home > Mouse Monoclonal Antibodies > RAD9A Monoclonal Antibody

RAD9A Monoclonal Antibody#27193

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Product Detail

Product NameRAD9A Monoclonal Antibody

Clone No.3A3-A7-F8

Host SpeciesMouse

ClonalityMonoclonal

IsotypeIgG2b

PurificationAffinity purified

ApplicationsWB IP

Species ReactivityHu Ms Rt Mk

SpecificityThis antibody detects endogenous levels of RAD9A,and does not cross-react with related proteins.

Immunogen TypeRecombinant Protein

Immunogen DescPurified recombinant human Rad9A protein fragments expressed in E.coli

Target NameRAD9A

ConjugateUnconjugated

Other NamesCell cycle checkpoint control protein; DNA repair exonuclease rad9 homolog A; hRAD 9; hRAD9; Rad 9; RAD 9A; RAD9 (S pombe) homolog; RAD9 homolog A; RAD9 homolog; RAD9A;

Accession NoUniprot: Q99638

Gene ID: 5883

Uniprot Q99638

Gene ID 5883;

Sdspage MW55kd

FormulationPurified mouse monoclonal in PBS(pH 7.4)containing with 0.2% sodium azide,50% glycerol.

Storagestore at -20˚C

Application Details

 Western blotting: 1:500

Western blot detection of RAD9A in Hela,MCF7,3T3,COS7&Rat testis cell lysates using RAD9A antibody(1:500 diluted).Predicted band size:43KDa,Observed band size:55KDa
Immunoprecipitation analysis of Hela & 3T3 cell lysates using RAD9A antibody

 Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair.The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C(RFC)clamp loader complex.Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair(LP-BER).The 9-1-1 complex stimulates DNA polymerase beta(POLB)activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds;endonuclease FEN1 cleavage activity on substrates with double,nick,or gap flaps of distinct sequences and lengths;and DNA ligase I(LIG1)on long-patch base excision repair substrates.The 9-1-1 complex is necessary for the recruitment of C12orf32/RHINO to sites of double-stranded breaks(DSB)occurring during the S phase.RAD9A possesses 3'->5' double stranded DNA exonuclease activity.Its phosphorylation by PRKCD may be required for the formation of the 9-1-1 complex.

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
;

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