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Location： Home > Mouse Monoclonal Antibodies > AMPK beta 1 Monoclonal Antibody

AMPK beta 1 Monoclonal Antibody#27201

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Product NameAMPK beta 1 Monoclonal Antibody

Clone No.1A7-E11-E9

Host SpeciesMouse

ClonalityMonoclonal

IsotypeHu Ms Rt Mk

PurificationAffinity purified

ApplicationsWB ICC IP IHC

Species ReactivityHu Ms Rt Mk

SpecificityThis antibody detects endogenous levels of AMPK beta 1 and does not cross-react with related proteins.

Immunogen TypeRecombinant Protein

Immunogen DescPurified recombinant human AMPK beta 1 protein fragments expressed in E.coli.

Target NameAMPK beta 1

ConjugateUnconjugated

Other Names1300015D22Rik; 5 AMP activated protein kinase subunit beta 1; 5''-AMP-activated protein kinase subunit beta-1; AAKB1_HUMAN; AMP-ACTIVATED PROTEIN KINASE, NONCATALYTIC, BETA-1; AMP-activated, noncatalytic, beta-1; AMPK; AMPK beta 1 chain;

Accession NoUniprot: Q9Y478

Gene ID: 5564

Uniprot Q9Y478

Gene ID 5564;

Sdspage MW38kd

FormulationPurified mouse monoclonal antibody in PBS(pH 7.4) containing with 0.02% sodium azide and 50% glycerol.

Storagestore at -20˚C

Application Details

Western blotting: 1:1000

Immunocytochemistry: 1:100
Immunocytochemistry staining of HeLa cells fixed with 1% Paraformaldehyde and using anti-AMPK beta 1 antibody (dilution 1:100).
Western blot detection of AMPK beta 1 in 3T3,Hela,PC-12,COS7 and MDA-MB-468 cell lysates using AMPK beta 1 antibody (1:1000 diluted).Predicted band size:38KDa.Observed band size:38KDa.Exposure time:5min.
Immunoprecipitation analysis of Hela cell lysates using AMPK beta 1 antibody.
Immunohistochemical analysis of paraffin-embedded Breast cancer using AMPK beta 1 Mouse mAb (1/200 dilution).Antigen retrieval was performed by pressure cooking in citrate buffer (pH 6.0).

 Non-catalytic subunit of AMP-activated protein kinase (AMPK),an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism.In response to reduction of intracellular ATP levels,AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein,carbohydrate and lipid biosynthesis,as well as cell growth and proliferation.AMPK acts via direct phosphorylation of metabolic enzymes,and by longer-term effects via phosphorylation of transcription regulators.Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton;probably by indirectly activating myosin.Beta non-catalytic subunit acts as a scaffold on which the AMPK complex assembles,via its C-terminus that bridges alpha (PRKAA1 or PRKAA2) and gamma subunits (PRKAG1,PRKAG2 or PRKAG3).

If you have published an article using product 27201, please notify us so that we can cite your literature.

et al,Blocking AMPK β1 myristoylation enhances AMPK activity and protects mice from high-fat diet-induced obesity and hepatic steatosis. In Cell Rep on 2022 Dec 20 by Katyayanee Neopane, Natalie Kozlov, et al..PMID:36543129 , (2022),
PMID: 36543129
et al,AMPKβ isoform expression patterns in various adipocyte models and in relation to body mass index.In Front Physiol on 2022 Aug 4 by Franziska Kopietz, Eva Degerman,et al..PMID:35991175 , (2022),
PMID: 35991175

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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