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Location： Home > Mouse Monoclonal Antibodies > Prominin-1 Monoclonal Antibody

Prominin-1 Monoclonal Antibody#27208

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Product Detail

Product NameProminin-1 Monoclonal Antibody

Clone No.6H10-F1-C11

Host SpeciesMouse

ClonalityMonoclonal

IsotypeIgG2a

PurificationAffinity purified

ApplicationsWB

Species ReactivityHu

SpecificityThis antibody detects endogenous levels of PROM1 and does not cross-react with related proteins.

Immunogen TypeRecombinant Protein

Immunogen DescPurified recombinant human PROM1 protein fragments expressed in E.coli.

Target NameProminin-1

ConjugateUnconjugated

Accession NoUniprot: O43490

Gene ID: 8842

Uniprot O43490

Gene ID 8842;

Sdspage MW133kd

FormulationPurified mouse monoclonal in PBS(pH 7.4) containing with 0.02% sodium azide,0.1mg/mlBSA and 50% glycerol.

Storagestore at -20˚C

Application Details

 Western blotting: 1:1000

Western blot detection of PROM1 antibody in CaCo2 cell lysate using PROM1 antibody (1:1000 diluted).Predicted band size:97KDa.Observed band size:133KDa
Western blot detection of PROM1 expression in CHO-k1 cells non-transfected (A) or transfected (B) with PROM1 and using PROM1 antibody (1:1000 diluted).Predicted band size:97KDa.Observed band size:133KDa.

 Binds cholesterol in cholesterol-containing plasma membrane microdomains.Proposed to play a role in apical plasma membrane organization of epithelial cells.During early retinal development acts as a key regulator of disk morphogenesis.Involved in regulation of MAPK and Akt signaling pathways.In neuroblastoma cells suppresses cell differentiation such as neurite outgrowth in a RET-dependent manner.

If you have published an article using product 27208, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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