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EGFP/EYFP Monoclonal Antibody#27209

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Product Detail

Product NameEGFP/EYFP Monoclonal Antibody

Clone No.3F11-B12-E7-F12

Host SpeciesMouse

ClonalityMonoclonal

IsotypeIgG1

PurificationAffinity purified

ApplicationsWB

Species ReactivityBacillus cereus

SpecificityTransfected

Immunogen TypeRecombinant Protein

Immunogen DescPurified recombinant EYFP.tag full length expressed in E.coli.

Target NameEGFP/EYFP

ConjugateUnconjugated

Other NamesGFP; Green Fluorescent Protein; enhanced Green Fluorescent Protein;

Accession NoUniprot: C8CHS1

Uniprot C8CHS1

Sdspage MWAccording

FormulationPurified mouse monoclonal in PBS(pH 7.4) containing with 0.02% sodium azide and 50% glycerol.

Storagestore at -20˚C

Application Details

 Western blotting: 1:1000

Western blot detection of EGFP expression in 293T cells transfected (A:mRFP) or transfected (B:EGFP fused protein;C:EGFP) using EGFP antibody(L:1/60000 diluted. M:1/20000 diluted. R:1/10000 diluted).
Western blot detection of EYFP expression in Rosetta E.coli cells induced by 0(A)or 0.1mM (B)IPTG using EGFP antibody(1:1000 diluted).Predicted band size: 30KDa Observed band size: 30KDa.
Western blot detection of EGFP expression in Hela cells non-transfected (A) or transfected (B) with pEGFP C1 using EGFP antibody(1:5000 diluted).Predicted band size: 34KDa Observed band size: 34KDa.

 The green fluorescent protein (GFP) is a protein isolated from the jellyfish Aequorea victoria composed of 238 amino acid residues that exhibits bright green fluorescence. GFP has become a very useful tool as a fusion protein that reports gene expression, traces cell lineages and defines subcellular protein localizations. Due to the potential for widespread usage and the evolving needs of researchers, many different mutants of GFP have been engineered. For example, EGFP contains the double-amino-acid substitution of Phe-64 to Leu and Ser-65 to Thr which result in  brighter green fluorescence. EYFP contains the four amino acid substitutions of Ser-65 to Gly; Val-68 to Leu; Ser-72 to Ala; and Thr-203 to Tyr. EYFP emits yellow fluorescence excited by green light.

If you have published an article using product 27209, please notify us so that we can cite your literature.

et al,Purification and characterization of a novel cell-penetrating carrier similar to cholera toxin chimeric protein.In Protein Expr Purif on 2017 Jan by Weiping Lin, Xi Zheng ,et al..PMID: 27234002 , (2017),
PMID: 27234002

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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