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Location： Home > Mouse Monoclonal Antibodies > PCNA Monoclonal Antibody

PCNA Monoclonal Antibody#27210

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Product Detail

Product NamePCNA Monoclonal Antibody

Clone No.2E1-G10-H10

Host SpeciesMouse

ClonalityMonoclonal

IsotypeIgG2b

ApplicationsWB IP ICC IHC

Species ReactivityHu Ms Mk Rt Hm

SpecificityThis antibody detects endogenous levels of PCNA and does not cross-react with related proteins.

Immunogen TypeRecombinant Protein

Immunogen DescPurified recombinant human PCNA protein fragments expressed in E.coli.

Target NamePCNA

ConjugateUnconjugated

Other NamesCyclin; Cyclin; DNA polymerase delta auxiliary protein; DNA polymerase delta auxiliary protein; HGCN8729; MGC8367; MGC8367; Mutagen-sensitive 209 protein; OTTHUMP00000030189; OTTHUMP00000030190; PCNA; Pcna/cyclin; PCNA_HUMAN; PCNAR;

Accession NoUniprot: P12004

Gene ID: 5111

Uniprot P12004

Gene ID 5111;

Sdspage MW36kd

Formulationascites

Storagestore at -20˚C

Application Details

Western blotting: 1:1000

Immunocytochemistry: 1:100
Immunocytochemistry staining of HeLa cells using anti-PCNA antibody (dilution 1:100).Fixed in 100% methanol for 2hr at -20℃.
Western blot detection of PCNA antibody in Hela,3T3,COS7,C6 and CHO-K1 cell lysates using PCNA antibody (1:1000 diluted).Predicted band size:36KDa.Observed band size:36KDa.
Immunoprecipitation analysis of Hela cell lysates using PCNA antibody.
Immunohistochemical analysis of paraffin-embedded human colorectal carcinoma with PCNA Mouse mAb (2E1-G10-H10,1:400 diluted),showing nuclear localization.A high pressure mediated antigen retrieval step was performed in citrate buffer(pH6.0).

 Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand.Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase,but not apurinic-apyrimidinic (AP) endonuclease,APEX2 activities.Has to be loaded onto DNA in order to be able to stimulate APEX2.Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways.Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair:Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases,while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion.

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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