Welcome to Sabbiotech!     
Email:info@signalwayantibody.com
Welcome Login Register My Cart(0,$0.00)
Location： Home > Mouse Monoclonal Antibodies > RPA70 Monoclonal Antibody

RPA70 Monoclonal Antibody#27212

Datasheet PDF
Availability： Yes
Total Amount:$
ADD TO CART
Detail Pictures Description Publish(0) Other
Product Detail

Product NameRPA70 Monoclonal Antibody

Clone No.8C3-D12-H10

Host SpeciesMouse

ClonalityMonoclonal

IsotypeIgG2a

PurificationAffinity purified

ApplicationsWB ICC IP

Species ReactivityHu Mk Ms Rt

SpecificityThis antibody detects endogenous levels of RPA70 and does not cross-react with related proteins.

Immunogen TypeRecombinant Protein

Immunogen DescPurified recombinant human RPA70 protein fragments expressed in E.coli.

Target NameRPA70

ConjugateUnconjugated

Other NamesDmrpa1; Drosophila Replication Protein A; DRPA; HSSB; Human single stranded DNA binding protein; MST075; MSTP075; p70; REPA1; Replication factor A; Replication factor A protein 1; Replication protein A 70 kDa DNA-binding subunit;

Accession NoUniprot: P27694

Gene ID: 6117

Uniprot P27694

Gene ID 6117;

Sdspage MW70kd

FormulationPurified mouse monoclonal antibody in PBS(pH 7.4) containing with 0.02% sodium azide and 50% glycerol.

Storagestore at -20˚C

Application Details

Western blotting: 1:1000

Immunocytochemistry: 1:100
Immunocytochemistry staining of HeLa cells fixed in 1% Paraformaldehyde and using anti-RPA70 antibody (dilution 1:100).
Western blot detection of RPA70 in Hela,A431,MCF7,COS7,HT1080 and K562 cell lysates using RPA70 antibody (1:1000 diluted).Predicted band size:70KDa.Observed band size:70KDa.
Western blot detection of RPA70 antibody in Hela,293T,C6,3T3 and K562 cell lysates using RPA70 antibody (1:1000 diluted).Predicted band size:70KDa.Observed band size:70KDa.
Western blot detection of RPA70 antibody in HUVEC,Hacat,3T3,MCF7 and A549 cell lysates using RPA70 antibody (1:2000 diluted).Predicted band size:70KDa.Observed band size:70KDa.
Immunoprecipitation analysis of Hela cell lysates using RPA70 antibody.

 Plays an essential role in several cellular processes in DNA metabolism including replication,recombination and DNA repair.Binds and subsequently stabilizes single-stranded DNA intermediates and thus prevents complementary DNA from reannealing.Functions as component of the alternative replication protein A complex (aRPA).aRPA binds single-stranded DNA and probably plays a role in DNA repair;it does not support chromosomal DNA replication and cell cycle progression through S-phase.In vitro,aRPA cannot promote efficient priming by DNA polymerase alpha but supports DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC),the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange.

If you have published an article using product 27212, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
;

QUICK NAV

New Products

Cell Signal Transduction

Cancer

Stem Cell

Neuroscience

Help and Support

Contact Us

Terms

Privacy Policy

support

Distributors