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Location： Home > Mouse Monoclonal Antibodies > WDR77 Monoclonal Antibody

WDR77 Monoclonal Antibody#27213

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Product Detail

Product NameWDR77 Monoclonal Antibody

Clone No.8A10-C10-E8

Host SpeciesMouse

ClonalityMonoclonal

IsotypeIgG1

PurificationAffinity purified

ApplicationsWB ICC

Species ReactivityHu Ms Rt

SpecificityThis antibody detects endogenous levels of WDR77 and does not cross-react with related proteins.

Immunogen TypeRecombinant Protein

Immunogen DescPurified recombinant human WDR77 protein fragments expressed in E.coli.

Target NameWDR77

ConjugateUnconjugated

Other Names2610312E17Rik; Androgen receptor cofactor p44; C79984; HKMT1069; MEP 50; MEP-50; MEP50; MEP50_HUMAN; Methylosome protein 50; MGC2722; Nbla10071; p44; p44/Mep50; RGD1310479; RP11 552M11.3; WD repeat containing protein 77; WD repeat domain 77;

Accession NoUniprot: Q9BQA1

Gene ID: 79084

Uniprot Q9BQA1

Gene ID 79084;

Sdspage MW42kd

FormulationPurified mouse monoclonal in PBS(pH 7.4) containing with 0.02% sodium azide and 50% glycerol.

Storagestore at -20˚C

Application Details

Western blotting: 1:1000

Immunocytochemistry: 1:100
Immunocytochemistry staining of HeLa cells fixed in 1% Paraformaldehyde and then permeabilized in 0.1% Triton X-100，next using anti-WDR77 antibody (dilution 1:100).
Western blot detection of WDR77 antibody in C6,3T3 and K562 cell lysates using WDR77 antibody (1:1000 diluted).Predicted band size:42KDa.Observed band size:42KDa.

 Non-catalytic component of the 20S PRMT5-containing methyltransferase complex,which modifies specific arginines to dimethylarginines in several spliceosomal Sm proteins and histones.This modification targets Sm proteins to the survival of motor neurons (SMN) complex for assembly into small nuclear ribonucleoprotein core particles.Might play a role in transcription regulation.The 20S PRMT5-containing methyltransferase complex also methylates the Piwi proteins (PIWIL1, PIWIL2 and PIWIL4),methylation of Piwi proteins being required for the interaction with Tudor domain-containing proteins and subsequent localization to the meiotic nuage.

If you have published an article using product 27213, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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