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BRAP Monoclonal Antibody#27218

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Product Detail

Product NameBRAP Monoclonal Antibody

Clone No.1E7-C9-D10-B10

Host SpeciesMouse

ClonalityMonoclonal

IsotypeIgG1

PurificationAffinity purified

ApplicationsWB

Species ReactivityHu

SpecificityThis antibody detects endogenous levels of BRAP and does not cross-react with related proteins.

Immunogen TypeRecombinant Protein

Immunogen DescPurified recombinant human BRAP protein fragments expressed in E.coli.

Target NameBRAP

ConjugateUnconjugated

Other Names3010002G07Rik; BRAP2; BRCA1 associated protein; EC 6.3.2.; Galectin 2 binding protein; IMP; Impedes mitogenic signal propagation; Renal carcinoma antigen NY REN 63; RING finger protein 52; RNF52; zgc:92894.

Accession NoUniprot: Q7Z569

Gene ID: 8315

Uniprot Q7Z569

Gene ID 8315;

Sdspage MW67kd

FormulationPurified mouse monoclonal antibody in PBS(pH 7.4) containing with 0.02% sodium azide and 50% glycerol.

Storagestore at -20˚C

Application Details

Western blotting: 1:1000

Western blot detection of BRAP in A549 and HUVEC cell lysates using BRAP antibody (1:500 diluted).Predicted band size:67KDa.Observed band size:67KDa.
Western blot analysis of extracts from CHO-K1 cells, transfected with a human pFLAG-CMV2-BRAP construct (A) or transfected with a human pFLAG-CMV2 construct (B), using BRAP antibody (1:1000 diluted).

Negatively regulates MAP kinase activation by limiting the formation of Raf/MEK complexes probably by inactivation of the KSR1 scaffold protein. Also acts as a Ras responsive E3 ubiquitin ligase that, on activation of Ras, is modified by auto-polyubiquitination resulting in the release of inhibition of Raf/MEK complex formation. May also act as a cytoplasmic retention protein with a role in regulating nuclear transport.

If you have published an article using product 27218, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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