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Location： Home > Mouse Monoclonal Antibodies > hHR23b Monoclonal Antibody

hHR23b Monoclonal Antibody#27219

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Product Detail

Product NamehHR23b Monoclonal Antibody

Clone No.5H1-A10-A7

Host SpeciesMouse

ClonalityMonoclonal

IsotypeIgG2b

PurificationAffinity purified

ApplicationsWB ICC IHC

Species ReactivityHu Ms Rt Mk Hm

SpecificityThis antibody detects endogenous levels of hHR23b and does not cross-react with related proteins.

Immunogen TypeRecombinant Protein

Immunogen DescPurified recombinant human hHR23b protein fragments expressed in E.coli.

Target NamehHR23b

ConjugateUnconjugated

Other Nameshomolog of B; RAD23B; RD23B_HUMAN; UV excision repair protein RAD23 homolog B; XP C repair complementing complex 58 kDa; XP C repair complementing complex 58 kDa protein; XP C repair complementing protein; XP-C repair-complementing complex 58 kDa protein;

Accession NoUniprot: P54727

Gene ID: 5887

Uniprot P54727

Gene ID 5887;

Sdspage MW58kd

FormulationPurified mouse monoclonal antibody in PBS(pH 7.4) containing with 0.02% sodium azide and 50% glycerol.

Storagestore at -20˚C

Application Details

Western blotting: 1:1000

Immunocytochemistry: 1:100

Immunocytochemistry staining of HeLa cells fixed with 4% Paraformaldehyde and using anti-hHR23b antibody (dilution 1:100).
Western blot detection of hHR23b in A431,K562,Jurkat,C6,3T3 and Hela cell lysates using hHR23b antibody (1:1000 diluted).Predicted band size:58KDa.Observed band size:58KDa.Exposure time:5min.
Immunohistochemical analysis of paraffin-embedded Prostate Cancer using hHR23b Mouse mAb (1/100 dilution).Antigen retrieval was performed by pressure cooking in citrate buffer (pH 6.0).
Western blot detection of hHR23b in K562,SW480,CHO-K1,3T3 and COS7 cell lysates using hHR23b antibody (1:1000 diluted).Predicted band size:58KDa.Observed band size:58KDa.Exposure time:5min.

Multiubiquitin chain receptor involved in modulation of proteasomal degradation. Binds to polyubiquitin chains. Proposed to be capable to bind simultaneously to the 26S proteasome and to polyubiquitinated substrates and to deliver ubiquitinated proteins to the proteasome. May play a role in endoplasmic reticulum-associated degradation (ERAD) of misfolded glycoproteins by association with PNGase and delivering deglycosylated proteins to the proteasome.
 

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
;

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