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HO-1 Polyclonal Antibody#27513

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Product Detail

Product NameHO-1 Polyclonal Antibody

Host SpeciesRabbit

ClonalityPolyclonal

IsotypeIgG

PurificationAffinity purification

ApplicationsWB,IHC,IF

Species ReactivityHuman,Mouse,Rat

Immunogen DescRecombinant fusion protein of human HO-1 (NP_002124.1).

ConjugateUnconjugated

Other NamesHMOX1; HMOX1D; HO-1; HSP32; bK286B10; heme oxygenase 1

Accession NoSwiss-Prot#:P09601
NCBI Gene ID:3162

Uniprot P09601

Gene ID 3162;

Calculated MW32kDa

FormulationAvoid freeze / thaw cycles.|Buffer: PBS with 50% glycerol, pH7.4.

StorageStore at -20˚C

Application Details
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Immunohistochemistry of paraffin-embedded rat lung using HO-1 at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human liver using HO-1 at dilution of 1:200 (40x lens).
Western blot analysis of extracts of various cell lines, using HO-1 at 1:1000 dilution.
Immunofluorescence analysis of HeLa cells using HO-1 at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Heme oxygenase, an essential enzyme in heme catabolism, cleaves heme to form biliverdin, which is subsequently converted to bilirubin by biliverdin reductase, and carbon monoxide, a putative neurotransmitter. Heme oxygenase activity is induced by its substrate heme and by various nonheme substances. Heme oxygenase occurs as 2 isozymes, an inducible heme oxygenase-1 and a constitutive heme oxygenase-2. HMOX1 and HMOX2 belong to the heme oxygenase family.

If you have published an article using product 27513, please notify us so that we can cite your literature.

et al,Classical prescription Daqinjiao decoction inhibit cerebral ischemia/reperfusion induced necroptosis and ferroptosis through multiple mechanisms. , (2025),
PMID: 39736347

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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