Product Detail
                                
                                                                        Product NameGPX4 Polyclonal Antibody
                                                                                 Host SpeciesRabbit
                                         ClonalityPolyclonal
                                        IsotypeIgG
                                        PurificationAffinity purification
                                        ApplicationsWB,IHC,IF
                                        Species ReactivityHuman,Mouse,Rat
                                                                                                                         Immunogen DescRecombinant fusion protein of human GPX4 (NP_002076.2).     
                                                                                 ConjugateUnconjugated
                                                                                 Other NamesGPX4;GPx-4;GSHPx-4;MCSP;PHGPx;SMDS;snGPx;snPHGPx
                                        Accession NoGeneID:2879
Swiss Prot:P36969
                                        Uniprot
                                        P36969
                                        
                                        Gene ID
                                        2879;
                                        
                                        Calculated MW19kDa/22kDa
                                        Sdspage MW25kDa
                                                                                 FormulationPBS with 0.02% sodium azide,50% glycerol,pH7.3.
                                        StorageStore at -20˚C. Avoid freeze / thaw cycles.|Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.240.
                                                                                                                                                         
                                Application Details
                                WB	1:500 - 1:2000
IHC	1:50 - 1:200
IF	1:50 - 1:200
                             
                            
                                                                
                                    
                                    Immunohistochemistry of paraffin-embedded human liver damage using GPX4 antibody  at dilution of 1:200 .
                                 
                                                                
                                    
                                    Immunofluorescence analysis of A549 cells using GPX4 antibody . Blue: DAPI for nuclear staining.
                                 
                                                                
                                    
                                    Western blot analysis of extracts of various cell lines, using GPX4 antibody  at 1:1000 dilution.
                                 
                                                             
                            
                                The protein encoded by this gene belongs to the glutathione peroxidase family, members of which catalyze the reduction of hydrogen peroxide, organic hydroperoxides and lipid hydroperoxides, and thereby protect cells against oxidative damage. Several isozymes of this gene family exist in vertebrates, which vary in cellular location and substrate specificity. This isozyme has a high preference for lipid hydroperoxides and protects cells against membrane lipid peroxidation and cell death. It is also required for normal sperm development; thus, it has been identified as a 'moonlighting' protein because of its ability to serve dual functions as a peroxidase, as well as a structural protein in mature spermatozoa. Mutations in this gene are associated with Sedaghatian type of spondylometaphyseal dysplasia (SMDS). This isozyme is also a selenoprotein, containing the rare amino acid selenocysteine (Sec) at its active site. Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal. Alternatively spliced transcript variants have been found for this gene. 
                            
                            
                                If you have published an article using product 29146, please notify us so that we can cite your literature.
                                                                                                    
                                      et al,Classical prescription Daqinjiao decoction inhibit cerebral ischemia/reperfusion induced necroptosis and ferroptosis through multiple mechanisms.
 ,  (2025),
PMID:
                                    39736347