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Location： Home > Polyclonal Antibodies > ZO-1 Rabbit Polyclonal Antibody

ZO-1 Rabbit Polyclonal Antibody#29274

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Product Detail

Product NameZO-1 Rabbit Polyclonal Antibody

Host SpeciesRabbit

ClonalityPolyclonal

IsotypeIgG

PurificationAffinity purification

ApplicationsWB,IHC,IF

Species ReactivityHuman,Mouse,Rat

Immunogen DescA synthetic peptide of human ZO-1 (NP_003248.3).

ConjugateUnconjugated

Other NamesTJP1;ZO-1;ZO1

Accession NoSwiss-Prot#:Q07157
NCBI Gene ID:7082

Uniprot Q07157

Gene ID 7082

Calculated MW186kDa/195kDa

FormulationPBS with 0.02% sodium azide,50% glycerol,pH7.3.

StorageStore at -20˚C. Avoid freeze / thaw cycles.

Application Details

WB 1:2000 - 1:5000

IHC 1:50 - 1:200

IF   1:50 - 1:200

Western blot analysis of extracts of various cell lines, using ZO-1 antibody.
Western blot analysis of extracts of Rat liver, using ZO-1 antibody.
Immunohistochemistry of paraffin-embedded Mouse stomach using ZO-1 antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded Rat stomach using ZO-1 antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunofluorescence analysis of MCF7 cells using ZO-1 Rabbit pAb at dilution of 1:150 (40x lens). Blue: DAPI for nuclear staining.

This gene encodes a protein located on a cytoplasmic membrane surface of intercellular tight junctions. The encoded protein may be involved in signal transduction at cell-cell junctions. Alternative splicing of this gene results in multiple transcript variants.

If you have published an article using product 29274, please notify us so that we can cite your literature.

et al,Hederacoside C ameliorates colitis via restoring impaired intestinal barrier through moderating S100A9/MAPK and neutrophil recruitment inactivation. In Acta Pharmacol Sin on 2023 Jan by Zheng-Xia Zha, Yu Lin, et al..PMID:35732707 , (2022),
PMID: 35732707
et al,Ephrin B3 exacerbates colitis and colitis-associated colorectal cancerInBiochem PharmacolOn2023 Dec 22byZhen Qiao?1,?Min Liao? et al..PMID: 38142837 , (2023),
PMID: 38142837
et al,Hederacoside C ameliorates colitis via restoring impaired intestinal barrier through moderating S100A9/MAPK and neutrophil recruitment inactivationInActa Pharmacol SinOn2023 JanbyZheng-Xia Zha?#?1,?Yu Lin et al..PMID:?35732707 , (2023),
PMID: 35732707
et al,Pulsatile Flow Increases METTL14-induced m6A modification and attenuates septic cardiomyopathy: an experimental study. In Int J Surg on 2024 Jul 1 by Shenyu Zhu, Kai Wang,et al..PMID:38549224 , (2024),
PMID: 38549224
et al,Ephrin B3 exacerbates colitis and colitis-associated colorectal cancer. In Biochem Pharmacol on 2024 Feb by Zhen Qiao, Min Liao,et al..PMID:38142837 , (2024),
PMID: 38142837
et al,Dynamic Impacts of Stock Enhancement on Kaluga Sturgeon (Huso dauricus): Novel Conservation Strategy Insights from the Gut Microbe Composition and Gene Expression Mode. , (2025),
PMID: 40003945
et al,Identification of Bioactive Compounds and Potential Mechanisms of Fuzi in the Treatment of Ulcerative Colitis by Integrating Network Pharmacology and Experimental Validation. , (2025),
PMID: 40101980
More
et al,Dictamnine alleviates DSS-induced colitis mice by inhibiting ferroptosis of enterocytes via activating Nrf2-Gpx4 signaling pathway. , (2025),
PMID: 40049578

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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