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VEGF Rabbit Polyclonal Antibody#29301

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Product Detail

Product NameVEGF Rabbit Polyclonal Antibody

Host SpeciesRabbit

ClonalityPolyclonal

IsotypeIgG

PurificationAffinity purification

ApplicationsWB IF/ICC

Species ReactivityHuman,Mouse,Rat

Immunogen DescRecombinant fusion protein of human VEGF (NP_001165099.1).

ConjugateUnconjugated

Other NamesVEGFA;MVCD1;VEGF;VPF;L VEGFA;VEGF A

Accession NoSwiss Prot:P15692
GeneID:7422

Calculated MW16kDa/20kDa/23kDa/26kDa

Concentration0.5 mg/ml

FormulationPBS with 0.09% Sodium azide,50% glycerol,pH7.3.

StorageStore at -20˚C. Avoid freeze / thaw cycles.

Application Details

WB 1:500 - 1:2000
IF/ICC 1:50 - 1:200

Western blot analysis of various lysates, using VEGFA Rabbit pAb at 1:1000 dilution.
Immunofluorescence - VEGFA Rabbit pAb Immunofluorescence analysis of NIH/3T3 cells using VEGFA Rabbit pAb at dilution of 100 (40x lens).

This gene is a member of the PDGF/VEGF growth factor family. It encodes a heparin-binding protein, which exists as a disulfide-linked homodimer. This growth factor induces proliferation and migration of vascular endothelial cells, and is essential for both physiological and pathological angiogenesis. Disruption of this gene in mice resulted in abnormal embryonic blood vessel formation. This gene is upregulated in many known tumors and its expression is correlated with tumor stage and progression. Elevated levels of this protein are found in patients with POEMS syndrome, also known as Crow-Fukase syndrome. Allelic variants of this gene have been associated with microvascular complications of diabetes 1 (MVCD1) and atherosclerosis. Alternatively spliced transcript variants encoding different isoforms have been described. There is also evidence for alternative translation initiation from upstream non-AUG (CUG) codons resulting in additional isoforms. A recent study showed that a C-terminally extended isoform is produced by use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism, and that this isoform is antiangiogenic. Expression of some isoforms derived from the AUG start codon is regulated by a small upstream open reading frame, which is located within an internal ribosome entry site.

If you have published an article using product 29301, please notify us so that we can cite your literature.

et al,Unraveling pathogenesis, biomarkers and potential therapeutic agents for endometriosis associated with disulfidptosis based on bioinformatics analysis, machine learning and experiment validation. In J Biol Eng on 2024 Jul 26 by Xiaoxuan Zhao, Yang Zhao,et al..PMID:39061076 , (2024),
PMID: 39061076

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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