Product Detail
Product NameAnti-SARS-CoV-2 Nucleocapsid antibody, Rabbit mAb
Clone No.DM22
Host SpeciesRabbit
ClonalityMonoclonal
IsotypeRabbit IgG
PurificationPurified from cell culture supernatant by affinity chromatography
ApplicationsELISA
Species ReactivitySARS-CoV-2
Immunogen DescRecombinant SARS-CoV-2 Nucleocapsid (Met1-Ala 419) produced by using E. coli
ConjugateUnconjugated
Other NamesSARS-CoV-2 Nucleocapsid
Calculated MW45kDa
FormulationPreservative: 0.1% Procline 300
Constituents: 50% Glycerol; PBS,pH 7.4; 0.1% BSA
StorageStore at -20˚C for 15 months (Avoid repeated freezing and thawing)
Application Details
ELISA 1/5000-10000
Figure 1. Elisa plate pre-coated by 2 μg/ml(100μl/well) SARS-CoV-2 Nucleocapsid protein, His Tag(Cat.No.PME100459) can bind Rabbit Anti-SARS-CoV-2 Nucleocapsid monoclonal antibody(clone:DM22) in a linear range of 0.24-62.5 ng/ml.
Coronavirus contain most of nucleocapsid protein. Coronavirus nucleoproteins (N proteins) localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. The nucleolus is the site of ribosome biogenesis and sequesters cell cycle regulatory complexes. Two of the major components of the nucleolus are fibrillarin and nucleolin. These proteins are involved in nucleolar assembly and ribosome biogenesis and act as chaperones for the import of proteins into the nucleolus. Regarding of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is a tool for diagnostic.
If you have published an article using product 29627, please notify us so that we can cite your literature.
et al,Unique Severe COVID-19 Placental Signature Independent of Severity of Clinical Maternal Symptoms
, (2021),
PMID:
34452534
et al,Similar Spatial Expression of Immune-Related Proteins in SARS-CoV-2 Placentitis and Chronic Histiocytic Intervillositis.
, (2025),
PMID:
39821970
Yes
