Product Detail
Product NameTRAF6 Antibody
Host SpeciesRabbit
ClonalityPolyclonal
ApplicationsELISA WB IHC
Species ReactivityHu
SpecificityThe antibody detects endogenous level of total TRAF6 protein.
Immunogen TypeRecombinant protein
Immunogen DescFusion protein corresponding to a region derived from 350-522 amino acids of human TNF receptor-associated factor 6, E3 ubiquitin protein ligase
Target NameTRAF6
ConjugateUnconjugated
Other NamesTNF receptor-associated factor 6, E3 ubiquitin protein ligase, RNF85; MGC:3310
FormulationRabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
StorageStore at -20˚C/1 year
Application Details
Predicted MW: 60kd
ELISA: 1:1000-1:2000
Western blotting: 1:200-1:1000
Immunohistochemistry: 1:15-1:50
Gel: 10%SDS-PAGE
Lane1: A431 cell lysate
Lane2: Human liver cancer tissue lysate
Lane3: Hela cell lysate
Lysates: 40 ug per lane
Primary antibody: 1/250 dilution
Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution
Exposure time: 2 minutes
The image on the left is immunohistochemistry of paraffin-embedded human thyroid cancer tissue using 31136(TRAF6 Antibody) at dilution 1/15, on the right is treated with the fusion protein.
The protein encoded by this gene is a member of the TNF receptor associated factor (TRAF) protein family. TRAF proteins are associated with, and mediate signal transduction from, members of the TNF receptor superfamily. This protein mediates signaling from members of the TNF receptor superfamily as well as the Toll/IL-1 family. Signals from receptors such as CD40, TNFSF11/RANCE and IL-1 have been shown to be mediated by this protein. This protein also interacts with various protein kinases including IRAK1/IRAK, SRC and PKCzeta, which provides a link between distinct signaling pathways. This protein functions as a signal transducer in the NF-kappaB pathway that activates IkappaB kinase (IKK) in response to proinflammatory cytokines. The interaction of this protein with UBE2N/UBC13, and UBE2V1/UEV1A, which are ubiquitin conjugating enzymes catalyzing the formation of polyubiquitin chains, has been found to be required for IKK activation by this protein. This protein also interacts with the transforming growth factor (TGF) beta receptor complex and is required for Smad-independent activation of the JNK and p38 kinases.
If you have published an article using product 31136, please notify us so that we can cite your literature.
et al,Classical prescription Daqinjiao decoction inhibit cerebral ischemia/reperfusion induced necroptosis and ferroptosis through multiple mechanisms.
, (2025),
PMID:
39736347
Yes
