Welcome to Sabbiotech!     
Email:info@signalwayantibody.com
Welcome Login Register My Cart(0,$0.00)
Location： Home > Polyclonal Antibodies > BCL2 Antibody

BCL2 Antibody#32012

Datasheet PDF
Availability： Yes
Total Amount:$
ADD TO CART
Detail Pictures Description Publish(3) Other
Product Detail

Product NameBCL2 Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were purified by affinity purification using immunogen.

ApplicationsWB,IHC,IF

Species ReactivityHuman,Mouse,Rat

SpecificityThe antibody detects endogenous level of total BCL2 protein.

Immunogen TypePeptide

Immunogen DescThe antiserum was produced against synthesized peptide derived from human BCL-2.

Target NameBCL2

ConjugateUnconjugated

Other NamesBCL2; Bcl-2; ONCOGENEB-CELLLEUKEMIA2; ApoptosisregulatorBcl-2;

Accession NoSwiss-Prot:P10415
NCBI Gene ID:596

Uniprot P10415

Gene ID 596;

Sdspage MW26KD

Concentration1.0mg/ml

FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

StorageStore at -20˚C

Application Details

WB 1:500 - 1:2000
IHC 1:100- 1:300
IF 1:200 - 1:1000

Western blot analysis of lysates from 1)mouse-liver, 2)mouse-brain, 3)mouse-lung, 4)mcf-7 cells, (Green)primary antibody was diluted at 1:1000, 4°over night, Dylight 800 secondary antibody was diluted at 1:10000, 37° 1hour. (Red) Actin β Monoclonal Antibody(5B7) antibody was diluted at 1:5000 as loading control, 4° over night,Dylight 680 secondary antibody was diluted at 1:10000, 37° 1hour.
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, using BCL-2 Antibody. The picture on the right is blocked with the synthesized peptide.
Immunofluorescence analysis of HepG2 cells, using BCL-2 Antibody. The picture on the right is blocked with the synthesized peptide.
Lane1: 3T3 whole cell lysateLane2:HepG2 whole cell lysateLane3:JK whole cell lysateLane4: MCF7 whole cell lysateLane5: Hela whole cell lysateLane6:293T whole cell lysateLane7:Mouse Brain tissue lysateLane8:Mouse Spleen tissue lysateLane9:Rat Kidney tissue lysateLane10: Rat Liver tissue lysateSecondary: Goat Anti-Rabbit IgG(HRP) at 1/20000 dilutionPredicted band size : 26kDaObserved band size: 26kDa

BCL2 exerts a survival function in response to a wide range of apoptotic stimuli through inhibition of mitochondrial cytochrome c release (1). It has been implicated in modulating mitochondrial calcium homeostasis and proton flux (2). Several phosphorylation sites have been identified within BCL2 including Thr56, Ser70, Thr74 and Ser87 (3). It has been suggested that these phosphorylation sites may be targets of the ASK1/MKK7/JNK1 pathway, and that phosphorylation of BCL2 may be a marker for mitotic events (4,5). Mutation of BCL2 at Thr56 or Ser87 inhibits its anti-apoptotic activity during glucocorticoid-induced apoptosis of T lymphocytes (6). Interleukin 3 and JNK-induced BCL2 phosphorylation at Ser70 may be required for its enhanced anti-apoptotic functions (7).

If you have published an article using product 32012, please notify us so that we can cite your literature.

et al,PIWI-Interacting RNA-004800 Is Regulated by S1P Receptor Signaling Pathway to Keep Myeloma Cell Survival.In Front Oncol on 2020 Apr 15 by Ma H, Wang H, et al..PMID: 32351883 , (2020),
PMID: 32351883
et al,Nerve growth factor protects retinal ganglion cells against injury induced by retinal ischemia鈥搑eperfusion in rats.In Growth Factors on 2015 Apr by Qian Chen , Haitao Wang et al..PMID: 25707536 , (2015),
PMID: 25707536
et al,Artemisinin Improved Neuronal Functions in Alzheimer's Disease Animal Model 3xtg Mice and Neuronal Cells via Stimulating the ERK/CREB Signaling Pathway. In Aging Dis on 2020 Jul 23 by Xia Zhao, Shuai Li,et al..PMID:32765947 , (2020),
PMID: 32765947

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
;

QUICK NAV

New Products

Cell Signal Transduction

Cancer

Stem Cell

Neuroscience

Help and Support

Contact Us

Terms

Privacy Policy

support

Distributors