Product Detail
Product NameGFAP Antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationAntibodies were purified by affinity purification using immunogen.
ApplicationsWB,IHC,IF
Species ReactivityHuman,Mouse,Rat
SpecificityThe antibody detects endogenous level of total GFAP protein.
Immunogen TypeRecombinant Protein
Immunogen DescRecombinant protein of human GFAP .
Target NameGFAP
ConjugateUnconjugated
Other NamesGFAP; FLJ45472;
Accession NoSwiss-Prot:P14136
NCBI Gene ID:2670
Uniprot
P14136
Gene ID
2670;
Sdspage MW50KD
Concentration1.0mg/ml
FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20˚C
Application Details
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Western blot analysis of extracts of various cell lines, using GFAP at 1:1000 dilution.
Immunohistochemistry of paraffin-embedded rat brain using GFAP at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded rat cerebellum using GFAP at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using GFAP at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U-251MG cells using GFAP at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of rat brain using GFAP at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of mouse brain using GFAP at dilution of 1:100. Blue: DAPI for nuclear staining.
The cytoskeleton consists of three types of cytosolic fibers: microfilaments (actin filaments), intermediate filaments, and microtubules. Major types of intermediate filaments are specifically expressed in particular cell types: cytokeratins in epithelial cells, glial fibrillary acidic protein (GFAP) in glial cells, desmin in skeletal, visceral, and certain vascular smooth muscle cells, vimentin in cells of mesenchymal origin, and neurofilaments in neurons. GFAP and vimentin form intermediate filaments in astroglial cells and modulate their motility and shape (1). In particular, vimentin filaments are present at early developmental stages, while GFAP filaments are characteristic of differentiated and mature brain astrocytes. Thus, GFAP is commonly used as a marker for intracranial and intraspinal tumors arising from astrocytes (2). In addition, GFAP intermediate filaments are also present in non-myelin-forming Schwann cells in the peripheral nervous system (3).
If you have published an article using product 32033, please notify us so that we can cite your literature.
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et al,Artemisinin alleviates astrocyte overactivation and neuroinflammation by modulating the IRE1/NF-κB signaling pathway in in vitro and in vivo Alzheimer's disease models.
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