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HK2 Antibody#32115

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Product Detail

Product NameHK2 Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were purified by affinity purification using immunogen.

ApplicationsWB,IHC,IF

Species ReactivityHuman,Mouse,Rat

SpecificityThe antibody detects endogenous level of total HK2 protein.

Immunogen TypeRecombinant Protein

Immunogen DescRecombinant protein of human HK2.

Target NameHK2

ConjugateUnconjugated

Other NamesHK2; DKFZp686M1669; HKII; HXK2;

Accession NoSwiss-Prot:P52789
NCBI Gene ID:3099

Uniprot P52789

Gene ID 3099;

Sdspage MW102KD

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20˚C

Application Details
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Immunohistochemistry of paraffin-embedded human breast cancer using HK2 at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human colon using HK2 at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using HK2 at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded rat heart using HK2 at dilution of 1:200 (40x lens).
Western blot analysis of extracts of various cell lines, using HK2 at 1:1000 dilution.
Immunofluorescence analysis of U2OS cells using HK2 . Blue: DAPI for nuclear staining.
Hexokinase catalyzes the conversion of glucose to glucose-6-phosphate, the first step in glycolysis. Four distinct mammalian hexokinase isoforms, designated as hexokinase I, II, III, and IV (glucokinase), have been identified. Hexokinases I, II, and III are associated with the outer mitochondrial membrane and are critical for maintaining an elevated rate of aerobic glycolysis in cancer cells (Warburg Effect) (1) in order to compensate for the increased energy demands associated with rapid cell growth and proliferation (2,3).

If you have published an article using product 32115, please notify us so that we can cite your literature.

et al,CircLARP4 Suppresses Cell Proliferation, Invasion and Glycolysis and Promotes Apoptosis in Non-Small Cell Lung Cancer by Targeting miR-135b. In Onco Targets Ther on 2020 May 4 by Huawei Lu, Qingwei Guo, et al..PMID:32440141 , (2020),
PMID: 32440141
et al,Multifunctional manganese-based nanogels catalyze immune energy metabolism to promote bone repair , (2025),
PMID:

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
;

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