Product Detail
Product NameCREB1 Antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationAntibodies were purified by affinity purification using immunogen.
ApplicationsWB,IHC,IF
Species ReactivityHuman,Mouse,Rat
SpecificityThe antibody detects endogenous level of total CREB1 protein.
Immunogen TypeRecombinant Protein
Immunogen DescRecombinant protein of human CREB1.
Target NameCREB1
ConjugateUnconjugated
Other NamesCREB1; CREB; MGC9284;
Accession NoSwiss-Prot:P16220
NCBI Gene ID:1385
Uniprot
P16220
Gene ID
1385;
Sdspage MW35KD
Concentration1.0mg/ml
FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20˚C
Application Details
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IP 1:50 - 1:100
Immunofluorescence analysis of HeLa cells using CREB1 at dilution of 1:100. Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using CREB1 at 1:3000 dilution.
CREB is a bZIP transcription factor that activates target genes through cAMP response elements. CREB is able to mediate signals from numerous physiological stimuli, resulting in regulation of a broad array of cellular responses. While CREB is expressed in numerous tissues, it plays a large regulatory role in the nervous system. CREB is believed to play a key role in promoting neuronal survival, precursor proliferation, neurite outgrowth, and neuronal differentiation in certain neuronal populations (1-3). Additionally, CREB signaling is involved in learning and memory in several organisms (4-6). CREB is able to selectively activate numerous downstream genes through interactions with different dimerization partners. CREB is activated by phosphorylation at Ser133 by various signaling pathways including Erk, Ca2+, and stress signaling. Some of the kinases involved in phosphorylating CREB at Ser133 are p90RSK, MSK, CaMKIV, and MAPKAPK-2 (7-9).
If you have published an article using product 32215, please notify us so that we can cite your literature.
et al,尾-Cryptoxanthin Suppresses UVB-induced Melanogenesis in Mouse: Involvement of the Inhibition of Prostaglandin E2 and Melanocyte-Stimulating Hormone Pathways. In J Pharm Pharmacol on 2012 Aug by Hiroshi Shimoda, Shao-Jie Shan, et al..PMID: 22775220
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PMID:
22775220
et al,Gene expression pattern in PC12 cells with reduced PMCA2 or PMCA3 isoform: selective up-regulation of calmodulin and neuromodulin. In Mol Cell Biochem on 2012 Jan by Tomasz Boczek, Anna Kozaczuk, et al..PMID: 21912933
, (2012),
PMID:
21912933
et al,Classical prescription Daqinjiao decoction inhibit cerebral ischemia/reperfusion induced necroptosis and ferroptosis through multiple mechanisms.
, (2025),
PMID:
39736347
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