Product Detail
Product NameHMOX1 Antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationAntibodies were purified by affinity purification using immunogen.
ApplicationsWB,IHC,IF
Species ReactivityHuman,Mouse,Rat
SpecificityThe antibody detects endogenous level of total HMOX1 protein.
Immunogen TypeRecombinant Protein
Immunogen DescRecombinant protein of human HMOX1.
Target NameHMOX1
ConjugateUnconjugated
Other NamesHO-1; HSP32; bK286B10; HMOX1;
Accession NoSwiss-Prot:P09601
NCBI Gene ID:3162
Uniprot
P09601
Gene ID
3162;
Sdspage MW33KD
Concentration1.0mg/ml
FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20˚C
Application Details
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Western blot analysis of extracts of various cell lines, using HO-1 at 1:1000 dilution.
Immunofluorescence analysis of HeLa cells using HO-1 Polyclonal at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunohistochemistry of paraffin-embedded human liver using HO-1 at dilution of 1:200 (40x lens).
Heme oxygenase (HO) is the rate-limiting enzyme in the catabolism of heme that results in the release of carbon monoxide, iron, and biliverdin (1). The products of this enzymatic reaction play important biological roles in antioxidant, anti-inflammatory and cytoprotective functions (2). Heme oxygenase comprises two isozymes, including the constitutively expressed HO-2 isozyme and the inducible HO-1 isozyme (3). Inducible HO-1 is expressed as an adaptive response to several stimuli, including heme, metals, and hormones (4). The induction of HO-1 has been implicated in numerous disease states, such as transplant rejection, hypertension, atherosclerosis, Alzheimer disease, endotoxic shock, diabetes, inflammation, and neurological disorders (1,5).
If you have published an article using product 32266, please notify us so that we can cite your literature.
et al,5-(3,4-Difluorophenyl)-3-(6-methylpyridIn-3-yl)-1,2,4-oxadiazole (DDO-7263), a novel Nrf2 activator targetIng braIn tissue, protects agaInst MPTP-Induced subacute ParkInson's disease In mice by InhibitIng the NLRP3 Inflammasome and protects PC12 cells agaInst oxidative stress. In Free Radic Biol Med on 2019 Apr; by Xu LL, Wu YF,et al..PMID:30615919
, (2019),
PMID:
30615919
et al,Fascia-Derived Stem Cells Enhance Fat Graft Retention by Promoting Vascularization through the HMOX1-HIF-1α Pathway
, (2024),
PMID:
et al,Fascia-derived stem cells enhance fat graft retention by promoting vascularization through the HMOX1-HIF-1α pathway.
, (2025),
PMID:
40001185
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