Product Detail
Product NameFH Antibody
Host SpeciesRabbit
ClonalityPolyclonal
IsotypeIgG
PurificationAffinity purification
ApplicationsWB,IHC,IF
Species ReactivityHuman,Mouse,Rat
SpecificityThe antibody detects endogenous level of total FH protein.
Immunogen TypeRecombinant Protein
Immunogen DescRecombinant fusion protein of human FH (NP_000134.2).
Target NameFH
ConjugateUnconjugated
Other NamesFH;FMRD;HLRCC;LRCC;MCL;MCUL1
Accession NoUniprot:P07954
GeneID:2271
Uniprot
P07954
Gene ID
2271
Sdspage MW50kDa
Concentration1.0mg/ml
FormulationPBS with 0.02% sodium azide,50% glycerol,pH7.3.
StorageStore at -20˚C. Avoid freeze / thaw cycles.
Application Details
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:100
Immunofluorescence analysis of U2OS cells using FH antibody.
Western blot analysis of extracts of various cell lines, using FH antibody.
The protein encoded by this gene is an enzymatic component of the tricarboxylic acid (TCA) cycle, or Krebs cycle, and catalyzes the formation of L-malate from fumarate. It exists in both a cytosolic form and an N-terminal extended form, differing only in the translation start site used. The N-terminal extended form is targeted to the mitochondrion, where the removal of the extension generates the same form as in the cytoplasm. It is similar to some thermostable class II fumarases and functions as a homotetramer. Mutations in this gene can cause fumarase deficiency and lead to progressive encephalopathy.
If you have published an article using product 32975, please notify us so that we can cite your literature.
et al,GlcNAcylation of fumarase maintains tumour growth under glucose deficiency.
, (2017),
PMID:
28628081
et al,PAK4 Phosphorylates Fumarase and Blocks TGFβ-Induced Cell Growth Arrest in Lung Cancer Cells. In Cancer Res on 2019 Apr 1 by Chen T, Wang T, et al..PMID:30683654
, (2019),
PMID:
30683654
et al,O-GlcNAcylation of Fumarase Maintains Tumour Growth Under Glucose Deficiency .In Nat Cell Biol ON 2017 Jul by Ting Wang , Qiujing Yu,et al..PMID: 28628081
, (2017),
PMID:
28628081
et al,Local generation of fumarate promotes DNA repair through inhibition of histone H3 demethylation.In Nat Cell Biol on 2015 Sep by Yuhui Jiang, Xu Qian et al..PMID:26237645
, (2015),
PMID:
26237645
Yes
