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Location： Home > Polyclonal Antibodies > PEK/PERK (Ab-981) Antibody

PEK/PERK (Ab-981) Antibody#33247

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Product NamePEK/PERK (Ab-981) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

ApplicationsWB IHC IF/ICC ELISA

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous levels of total PEK/PERK protein.

Immunogen TypePeptide

Immunogen DescSynthesized non-phosphopeptide derived from human PEK/PERK around the phosphorylation site of threonine 981 (R-H-T(p)-G-Q).

Target NamePEK/PERK

ConjugateUnconjugated

Other NamesE2AK3; EC 2.7.11.1; EIF2AK3; Eukaryotic translation initiation factor 2-alpha kinase 3 precursor; HsPEK

Accession NoSwiss-Prot: Q9NZJ5
NCBI Gene ID: 9451

Uniprot Q9NZJ5

Gene ID 9451;

Sdspage MW125kd

Concentration1.0mg/ml

FormulationRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20˚C

Application Details

Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100

IF/ICC 1:100-1:500

ELISA 1:20000-1:40000

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using PEK/PERK (Ab-981) antibody #33247.
Western blot analysis of extracts from MCF-7 cells, using PEK/PERK (Ab-981) antibody #33247.
Western blot analysis of extracts from JK cells (Lane 2), using PEK/PERK (Ab-981) antiobdy #33247. The lane on the left is treated with synthesized peptide.

Phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2 (EIF2), leading to its inactivation and thus to a rapid reduction of translational initiation and repression of global protein synthesis. Serves as a critical effector of unfolded protein response (UPR)-induced G1 growth arrest due to the loss of cyclin-D1 (CCND1) By similarity.

Shi Y., J. Biol. Chem. 274:5723-5730(1999).
Sood R., Biochem. J. 346:281-293(2000).
Delepine M., Nat. Genet. 25-406-409(2000).

If you have published an article using product 33247, please notify us so that we can cite your literature.

et al,Quercetin Stimulates Mitochondrial Apoptosis Dependent on Activation of Endoplasmic Reticulum Stress in Hepatic Stellate Cells .In Pharm Biol. On 2016 Dec by Liwei He , Xianbang Hou et al..PMID:27572285 , (2016),
PMID: 27572285
et al,5鈥慛itro锟?锟?3鈥憄henylpropylamino) benzoic acid induces apoptosis of human lens epithelial cells via reactive oxygen species and endoplasmic reticulum stress through the mitochondrial apoptosis pathway. In Int J Mol Med on 2021 Apr by Lingzhi Niu, Xin Liu,et al..PMID:33604681 , (2021),
PMID: 33604681
et al,Qing Hua Chang Yin ameliorates chronic colitis in mice by inhibiting PERK–ATF4–CHOP pathway of ER stress and the NF-κB signalling pathway. In Pharm Biolon 2024 Dec by Liya Liu, Youqin Chen,et al..PMID:39034914 , (2024),
PMID: 39034914

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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